Meiotic cohesin-based chromosome structure is essential for homologous chromosome pairing in Schizosaccharomyces pombe.
Meiotic cohesin-based chromosome structure is essential for homologous chromosome pairing in Schizosaccharomyces pombe.
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DOI：
10.1007/s00412-015-0551-8
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发表时间：
2016-06
期刊：
影响因子：
1.6
通讯作者：
Hiraoka Y
中科院分区：
文献类型：
--
作者：
Ding DQ;Matsuda A;Okamasa K;Nagahama Y;Haraguchi T;Hiraoka Y
染色体结构在进入减数分裂时发生显着变化，以建立成功进行减数分裂特定事件所必需的染色体结构。早期的减数分裂事件涉及到在染色体的大多数部分用减数分裂的等价物替换非SMC有丝分裂粘附素，在减数分裂染色体上形成一个轴。我们先前已经证明，在裂殖酵母的减数分裂前期，减数分裂粘附素复合体是染色体紧凑所必需的。这些研究表明，在没有减数分裂粘蛋白亚基Rec8的情况下，染色体会被拉长，在没有粘连蛋白相关蛋白Pds5的情况下，染色体会被缩短。在这项研究中，我们利用超分辨率结构照明显微镜发现，Rec8在染色体上形成了一个线性轴，这是减数分裂前期染色质有组织的轴结构所必需的。在没有Pds5的情况下，Rec8轴变短，而染色体变宽。在Rec8或Pds5突变体中，同源染色体配对的频率降低。因此，Rec8和Pds5在构建支持同源染色体空间比对所需的染色体结构的平台中起着至关重要的作用。
Chromosome structure is dramatically altered upon entering meiosis to establish chromosomal architectures necessary for the successful progression of meiosis-specific events. An early meiotic event involves the replacement of the non-SMC mitotic cohesins with their meiotic equivalents in most part of the chromosome, forming an axis on meiotic chromosomes. We previously demonstrated that the meiotic cohesin complex is required for chromosome compaction during meiotic prophase in the fission yeast Schizosaccharomyces pombe. These studies revealed that chromosomes are elongated in the absence of the meiotic cohesin subunit Rec8 and shortened in the absence of the cohesin-associated protein Pds5. In this study, using super-resolution structured illumination microscopy, we found that Rec8 forms a linear axis on chromosomes, which is required for the organized axial structure of chromatin during meiotic prophase. In the absence of Pds5, the Rec8 axis is shortened whereas chromosomes are widened. In rec8 or pds5 mutants, the frequency of homologous chromosome pairing is reduced. Thus, Rec8 and Pds5 play an essential role in building a platform to support the chromosome architecture necessary for the spatial alignment of homologous chromosomes.