Anti-idiotype-induced, lipopolysaccharide-specific antibody response to Pseudomonas aeruginosa. II. Isotype and functional activity of the anti-idiotype-induced antibodies.
Anti-idiotype-induced, lipopolysaccharide-specific antibody response to Pseudomonas aeruginosa. II. Isotype and functional activity of the anti-idiotype-induced antibodies.
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针对铜绿假单胞菌的抗独特型诱导的脂多糖特异性抗体反应。
DOI：
10.4049/jimmunol.146.1.188
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发表时间：
1991
影响因子：
4.4
通讯作者：
M. Patawaran
中科院分区：
文献类型：
--
作者：
J. Schreiber;K. Nixon;M. Tosi;G. Pier;M. Patawaran
我们最近开发了一种鼠抗独特型单克隆抗体，其在体外作为铜绿假单胞菌 LPS 的 O 特异性多糖侧链 (Ps) 的分子模拟物，并在同系 BALB/c ByJ 小鼠中诱导 Ps 特异性抗体。在目前的研究中，我们证明这些抗Id诱导的Ps特异性抗体将补体固定在细菌细胞表面，并保护中性粒细胞减少小鼠免受致命的铜绿假单胞菌败血症。然而，抗 Id 诱导抗体的同种型分布类似于给小鼠施用纯化的 Ps 后观察到的限制模式（IgM 和 IgG3）。通过将抗 Id 与匙孔血蓝蛋白缀合，可以增强产生的 Ps 特异性抗体的免疫原性和同种型数量。最后，在用纯化的 Ps 免疫之前施用抗 Id，引发 BALB/c ByJ 小鼠以产生其他 Ps 特异性抗体同种型 (IgG1)。这些研究表明，这种抗 Id 在同系小鼠中诱导功能性抗 Ps 抗体，并且当以缀合物形式使用或作为 Ps 免疫前的引发剂时，产生与“T 细胞依赖性”一致的抗体反应。这些免疫策略可能有助于在人体中诱导多糖特异性抗体。
We recently developed a murine anti-idiotypic mAb that functioned as a molecular mimic of the O-specific polysaccharide side chain (Ps) of Pseudomonas aeruginosa LPS in vitro, and which induced Ps-specific antibodies in syngeneic BALB/c ByJ mice. In the current studies, we demonstrate that these anti-Id-induced, Ps-specific antibodies fix complement to the bacterial cell surface, and protect neutropenic mice from fatal P. aeruginosa sepsis. The isotypic distribution of the anti-Id-induced antibodies, however, resembles the restricted pattern (IgM and IgG3) seen after administration of purified Ps to mice. The immunogenicity and number of isotypes of Ps-specific antibody produced could be enhanced by conjugating the anti-Id to keyhole limpet hemocyanin. Finally, the anti-Id administered before immunization with purified Ps, primed BALB/c ByJ mice for production of other Ps-specific antibody isotypes (IgG1). These studies show that this anti-Id induces functional anti-Ps antibodies in syngeneic mice, and when used in conjugate form or as a priming agent before Ps immunization, yields an antibody response consistent with "T cell dependence." These immunization strategies may be useful for the induction of polysaccharide-specific antibodies in man.