Non-homologous end joining often uses microhomology: implications for alternative end joining.
Non-homologous end joining often uses microhomology: implications for alternative end joining.
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DOI：
10.1016/j.dnarep.2014.02.006
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发表时间：
2014-05
期刊：
影响因子：
3.8
通讯作者：
Lieber MR
中科院分区：
文献类型：
--
作者：
Pannunzio NR;Li S;Watanabe G;Lieber MR
Artemis和PALF（也称为APLF）似乎是参与NHEJ的主要核酸酶之一，并负责NHEJ中的大多数溶核末端加工。约60%的NHEJ事件显示DNA末端的比对，其在两个DNA末端之间使用1或2bp的微同源性（MH）。因此，MH是NHEJ的共同特征。对于大多数自然发生的人类染色体缺失（例如，氧化损伤或辐射后）和易位（如在人类肿瘤中所见的那些）以及遗传性染色体结构变异，MH的使用以NHEJ的典型频率发生，并且不表明需要更广泛MH的替代途径的主要参与。虽然我们主要关注人类NHEJ在DSB，比较这些点与其他真核生物，主要是S。cerevisiae，是信息。
Artemis and PALF (also called APLF) appear to be among the primary nucleases involved in NHEJ and responsible for most nucleolytic end processing in NHEJ. About 60% of NHEJ events show an alignment of the DNA ends that uses 1 or 2 bp of microhomology (MH) between the two DNA termini. Thus, MH is a common feature of NHEJ. For most naturally-occurring human chromosomal deletions (e.g., after oxidative damage or radiation) and translocations, such as those seen in human neoplasms and as well as inherited chromosomal structural variations, MH usage occurs at a frequency that is typical of NHEJ, and does not suggest major involvement of alternative pathways that require more extensive MH. Though we mainly focus on human NHEJ at DSBs, comparison on these points to other eukaryotes, primarily S. cerevisiae, is informative.