Bombyx mori phenolamine receptors : a comparative molecular biological study
Bombyx mori phenolamine receptors : a comparative molecular biological study
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家蚕酚胺受体：比较分子生物学研究
DOI：
10.1584/jpestics.r07-09
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发表时间：
2008
影响因子：
2.4
通讯作者：
Jia Huang
中科院分区：
文献类型：
--
作者：
Y. Ozoe;Jia Huang
从家蚕中克隆了两种生物胺受体的cDNA，并在HEK-293细胞中稳定表达;这些受体被鉴定为α-肾上腺素能样章鱼胺（OA）受体和酪胺（TA）受体，前者与细胞内Ca 2+和cAMP水平升高有关，后者与细胞内cAMP产生减弱有关。通过定点突变产生的突变体受体的功能测定，鉴定了OA和TA受体与OA和TA相互作用的相应氨基酸残基。基于三种构象状态的假设，提出了OA受体的激活机制。
The cDNAs coding for two biogenic amine receptors were cloned from the silkworm Bombyx mori and stably expressed in HEK-293 cells; these receptors were identified as an α-adrenergic-like octopamine (OA) receptor, which is linked to elevations of intracellular Ca 2+ and cAMP levels, and a tyramine (TA) receptor, which is linked to an attenuation of intracellular cAMP production. The respective amino acid residues ofOA and TA receptors involved in interactions with OA and TA were identified by functional assays of mutant receptors generated by site-directed mutagenesis. An activation mechanism of the OA receptor was proposed based on the assumption of three conformational states.
影响因子：
2.9
作者：
Huang, Jia;Hamasaki, Tomohiro;Ozoe, Yoshihisa
通讯作者：
Ozoe, Yoshihisa