[3H]morphine binding is enhanced by IL-1-stimulated thymocyte proliferation.
[3H]morphine binding is enhanced by IL-1-stimulated thymocyte proliferation.
复制标题
IL-1刺激的胸腺细胞增殖增强[3H]吗啡结合。
DOI：
10.1016/0014-5793(91)80023-v
复制
发表时间：
1991
期刊：
影响因子：
3.5
通讯作者：
Loh,HH
中科院分区：
文献类型：
--
作者：
Roy,S;Ge,BL;Ramakrishnan,S;Lee,NM;Loh,HH
通过[3 H]胸苷掺入测定，在存在植物血凝素（PHA）的情况下，用浓度递增的白细胞介素-1（IL-1）体外孵育的小鼠胸腺细胞显示出细胞增殖的剂量依赖性增加。在这些条件下，特异性[3 H]吗啡结合呈平行剂量依赖性增加，最大增加约为基础水平的5倍。结合位点不同于经典阿片受体，因为它们不是立体选择性的。白细胞介素-2在促进细胞增殖或增强阿片结合方面无效，但IL-1的作用可以被佛波醇肉豆蔻酸酯（PMA）模拟，这表明酪氨酸磷酸化的参与。这些结果表明，免疫细胞上的吗啡结合位点可以通过细胞因子活化来调节。
Mouse thymocytes incubated in vitro with increasing concentrations of interleukin‐1 (IL‐1) in the presence of phytohemagglutinin (PHA) exhibited a dose‐dependent increase in cell proliferation, as measured by [3H]thymidine incorporation. Under these conditions, there was a parallel dosedependent increase in specific [3H]morphine binding, with a maximum increase of approximately 5‐fold over basal levels. The binding sites differ from classical opioid receptors in that they are not stereo‐selective. Interleukin‐2 was ineffective in promoting either cell proliferation or enhanced opioid binding, but the effects of IL‐1 could be mimicked by phorbol myristate acetate (PMA), suggesting the involvement of tyrosine phosphorylation. These results indicate that morphine‐binding sites on immune cells can be regulated by cytokine activation.