Fluorophotometric measurement of the precorneal residence time of topically applied hyaluronic acid
Fluorophotometric measurement of the precorneal residence time of topically applied hyaluronic acid
复制标题
DOI：
10.1136/bjo.2007.121533
复制
发表时间：
2008-01-01
影响因子：
4.1
通讯作者：
Nishida, T.
中科院分区：
文献类型：
--
作者：
Mochizuki, H.;Yamada, M.;Nishida, T.
目的：本研究分别评估透明质酸在眼表面的作用，以及透明质酸在眼表面的作用。方法：采用0.1%透明质酸（HA）溶液溶解荧光素钠和0.1%荧光素-透明质酸（FHA）溶液溶解荧光素钠两种不同的荧光染料。将20 μ l的测试溶液涂抹在10名健康志愿者的眼睛上。荧光素钠溶解于生理盐水中作为对照。每分钟在角膜中央测量角膜前泪膜的荧光强度，持续10min。周转率是用半对数图中荧光强度随时间变化的方程来计算的，用%/min表示。结果：0.1% F-HA、0.1% HA和生理盐水的周转率分别为8.1 (SD 3.6)%/min、21.6 (2.8)%/min和31.0 (3.7)%/min。F-HA的周转率显著低于HA和生理盐水（p = 0.00012和p = 0.00000022； Mann-Whitney检验）。HA的周转率显著低于生理盐水（p = 0.00001； Mann-Whitney检验）。结论：我们的研究结果表明，应用透明质酸的水体积流量和透明质酸本身的周转是不同的。透明质酸分子可以通过表面化学和/或生化特性附着在眼表面。透明质酸在眼表面的长时间滞留可以解释透明质酸在几小时内增强泪膜稳定性的机制。
Purpose: This study was performed to separately assess the aqueous flow applied with hyaluronic acid, and the behaviour of hyaluronic acid itself on the ocular surface.Methods: Two different fluorescent dyes, fluorescein sodium dissolved in 0.1% hyaluronic acid (HA) solution and 0.1% fluorescein conjugated with hyaluronic acid (FHA) dissolved in saline, were used. A volume of 20 mu l of tested solution was applied to the eye of 10 healthy volunteers. Fluorescein sodium dissolved in saline served as a control. The fluorescent intensity of the precorneal tear film was measured at the central cornea every minute for 10 min. The turnover rate was calculated using the equation that plots fluorescent intensity against time in a semilog plot and expressed as %/min.Results: Turnover rates of topically applied 0.1% F-HA, 0.1% HA and saline were 8.1 (SD 3.6)%/min, 21.6 (2.8)%/min, and 31.0 (3.7)%/min, respectively. The turnover rate of F-HA was significantly lower than those of HA and saline (p = 0.00012 and p = 0.00000022, respectively; Mann-Whitney test). The turnover rate of HA was significantly lower than that of saline (p = 0.00001; Mann-Whitney test).Conclusion: Our results indicate that the bulk aqueous flow applied with HA and the turnover of HA itself are different. HA molecules may adhere to the ocular surface by surface-chemical and/or biochemical properties. The long retention time of HA on the ocular surface may explain the mechanism in which hyaluronic acid has been shown to enhance tear film stability for a few hours.