Redundant roles for the TFIID and SAGA complexes in global transcription
Redundant roles for the TFIID and SAGA complexes in global transcription
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DOI：
10.1038/35015104
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发表时间：
2000-06-08
期刊：
影响因子：
64.8
通讯作者：
Young, RA
中科院分区：
文献类型：
--
作者：
Lee, TI;Causton, HC;Young, RA
转录因子TFIID和SAGA是参与RNA聚合酶ii转录的多亚基复合物1,2。TFIID和SAGA含有共同的塔塔结合蛋白（TBP）相关因子（TAF(II)）亚基，每个复合物都含有一个具有组蛋白乙酰转移酶活性的亚基(3)。这些观察结果提出了关于这两种复合物在体内的功能是独特的还是重叠的问题。在这里，我们使用全基因组表达分析来研究酵母基因组的表达如何依赖于这两个复合物的共享和独特亚基。我们发现大多数基因的表达需要一个或多个常见的TAF（II）亚基，这表明TFIID和SAGA的功能是基因表达所广泛需要的。在TFIID和SAGA共有的亚基中，有三个类似组蛋白的TAF(II)s，它们被认为可以形成亚复合物并介导全局转录的共同功能。出乎意料的是，我们发现类似组蛋白的TAF(II)s在酵母基因组的表达中具有不同的作用。最重要的是，我们发现TFIID和SAGA的组蛋白乙酰化酶组分（TAF(II)145和Gcn5）在功能上是冗余的，这表明大部分酵母基因的表达可以通过这两种复合物的作用来调节。
The transcription factors TFIID and SAGA are multi-subunit complexes involved in transcription by RNA polymerase II1,2. TFIID and SAGA contain common TATA-binding protein (TBP)-associated factor (TAF(II)) subunits and each complex contains a subunit with histone acetyltransferase activity(3). These observations have raised questions about whether the functions of the two complexes in vivo are unique or overlapping. Here we use genome-wide expression analysis to investigate how expression of the yeast genome depends on both shared and unique subunits of these two complexes. We find that expression of most genes requires one or more of the common TAF(II) subunits, indicating that the functions of TFIID and SAGA are widely required for gene expression. Among the subunits shared by TFIID and SAGA are three histone-like TAF(II)s, which have been proposed to forma sub-complex and mediate a common function in global transcription. Unexpectedly, we find that the histonelike TAF(II)s have distinct roles in expression of the yeast genome. Most importantly, we show that the histone acetylase components of TFIID and SAGA (TAF(II)145 and Gcn5) are functionally redundant, indicating that expression of a large fraction of yeast genes can be regulated through the action of either complex.