Recombinant vaccines based on translocated effector proteins of Salmonella Pathogenicity Island 2
Recombinant vaccines based on translocated effector proteins of Salmonella Pathogenicity Island 2
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DOI：
10.1016/j.vaccine.2005.11.020
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发表时间：
2007-01-02
期刊：
影响因子：
5.5
通讯作者：
Hensel, Michael
中科院分区：
文献类型：
--
作者：
Husseiny, Mohamed I.;Wartha, Florian;Hensel, Michael
减毒活肠沙门氏菌是用于递送疫苗接种的异源抗原的有用载体。沙门氏菌 III 型分泌系统 (T3SS) 易位的效应蛋白已成功用于抗原递送。在这里，我们研究了沙门氏菌致病岛 2 (SPI2) 编码的 T3SS 效应蛋白的用途。我们观察到效应蛋白SseF适合递送各种具有异源抗原的融合蛋白。这种载体蛋白的效率在使用带有单核细胞增多性李斯特菌保护性抗原的融合蛋白的疫苗接种实验中得到了证明。因此，SseF 可用作异源蛋白易位用于疫苗接种的多功能载体。 (c) 2005 Elsevier Ltd. 保留所有权利。
Attenuated live Salmonella enterica are useful carriers for the delivery of heterologous antigens for vaccination. Effector proteins translocated by type III secretion systems (T3SS) of Salmonella have been successfully utilized for antigen delivery. Here we investigated the use of effector proteins of the T3SS encoded by Salmonella Pathogenicity Island 2 (SPI2). We observed that the effector protein SseF is suitable for delivery of various fusion proteins with heterologous antigens. The efficiency of this carrier protein was demonstrated in vaccination experiments with fusion proteins with Listeria monocytogenes protective antigens. SseF can thus be used as a versatile vehicle for translocation of heterologous proteins for vaccination. (c) 2005 Elsevier Ltd. All rights reserved.