LOCALIZATION OF NEUROTROPHIN RECEPTORS IN OLFACTORY EPITHELIUM AND BULB
LOCALIZATION OF NEUROTROPHIN RECEPTORS IN OLFACTORY EPITHELIUM AND BULB
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DOI：
10.1097/00001756-199312000-00030
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发表时间：
1993-12-13
期刊：
影响因子：
1.7
通讯作者：
RISLING, M
中科院分区：
文献类型：
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作者：
DECKNER, ML;FRISEN, J;RISLING, M
我们采用原位杂交技术对大鼠和猫嗅球中trk、trkB和trkC mRNA进行了定位。编码截断trkB受体的mRNA表达在所有层中都可见，而只有非常适度的全长trkB表达可以被检测到。trkC杂交在各层可见，在二尖瓣细胞层最密集。用免疫组织化学方法检测全长酪氨酸激酶trkB受体在嗅球和上皮中的定位。在嗅丝、上皮和离体嗅感觉神经元中可见trkb样免疫反应性。由于BDNF由嗅球内的嗅感觉神经元靶细胞表达，这些数据表明BDNF可能在初级嗅觉系统中作为靶源神经营养因子。
WE used in situ hybridization to localize trk, trkB and trkC mRNA, in rat and cat olfactory bulb. Expression of mRNA encoding truncated trkB receptors was seen in all layers, while only very modest full-length trkB expression could be detected. trkC hybridization was seen in all layers, most dense in the mitral cell layer. The localization of full-length tyrosine kinase trkB receptor in olfactory bulb and epithelium was examined with immunohistochemistry. trkB-like immunoreactivity was seen in the fila olfactoria, epithelium and in vitro, in olfactory sensory neurones. Since BDNF is expressed by olfactory sensory neurone target cells in the olfactory bulb, these data suggest that BDNF may act as a target derived neurotrophic factor in the primary olfactory system.