Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells
Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells
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使用细菌人工染色体（BAC）克隆进行荧光原位杂交分析中国仓鼠卵巢细胞染色体重排
DOI：
10.1016/j.ymeth.2011.11.002
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发表时间：
2012
期刊：
影响因子：
4.8
通讯作者：
and Takeshi Omasa
中科院分区：
文献类型：
--
作者：
Yihua Cao;Shuichi Kimura;Takayuki Itoi;Kohsuke Honda;Hisao Ohtake;and Takeshi Omasa
利用中国仓鼠卵巢（CHO）基因组细菌人工染色体（BAC）克隆进行染色体鉴定，有助于分析和了解CHO细胞系的染色体不稳定性，并有助于在构建重组CHO细胞过程中提高对染色体组织的认识。以BAC克隆为探针的荧光原位杂交成像技术（BAC- fish）可为染色体鉴定提供有价值的信息。在本研究中，我们使用BAC-FISH方法鉴定了CHO细胞中的染色体并分析了染色体重排。
Chromosome identification using Chinese hamster ovary (CHO) genomic bacterial artificial chromosome (BAC) clones has the potential to contribute to the analysis and understanding of chromosomal instability of CHO cell lines and to improve our understanding of chromosome organization during the establishment of recombinant CHO cells. Fluorescence in situ hybridization imaging using BAC clones as probes (BAC-FISH) can provide valuable information for the identification of chromosomes. In this study, we identified chromosomes and analyzed the chromosome rearrangement in CHO cells using BAC-FISH methods.