Rational design and efficacy of a multi-epitope recombinant protein vaccine against foot-and-mouth disease virus serotype A in pigs
Rational design and efficacy of a multi-epitope recombinant protein vaccine against foot-and-mouth disease virus serotype A in pigs
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猪口蹄疫病毒A血清型多表位重组蛋白疫苗的合理设计及功效
DOI：
10.1016/j.antiviral.2017.01.023
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发表时间：
2017-04-01
影响因子：
7.6
通讯作者：
Liu, Zaixin
中科院分区：
文献类型：
--
作者：
Cao, Yimei;Li, Dong;Liu, Zaixin
口蹄疫（FMD）是偶蹄动物的一种高度传染性疾病，这种疾病的爆发往往会造成经济灾难。近期，包括中国在内的多国发生一系列A型口蹄疫疫情。因此，开发安全有效的疫苗十分必要。我们设计了具有不同三维结构的多表位重组蛋白A6、A7和A8，并比较了它们在猪中的免疫原性。结果表明，A8对猪抵抗FMDV A血清型攻击具有最大的保护作用，因此选择A8作为疫苗抗原。我们进一步测试了 CpG DNA 与 A8 疫苗结合的佐剂活性，结果显示，与单独接种 A8 的猪相比，同时接种 A8 和 CpG 的猪的抗原特异性 IFN-γ 反应显着增加。疫苗效价测试表明，CpG佐剂的A8疫苗每剂对猪的保护剂量为10.81 50%（PD50），表明该疫苗有可能用于预防猪口蹄疫病毒A血清型感染的紧急疫苗接种活动。 (C) 2017 Elsevier B.V. 保留所有权利。
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals, and outbreaks of this disease are often economically catastrophic. Recently, a series of outbreaks of foot-and-mouth disease virus (FMDV) serotype A occurred in many countries, including China. Therefore, it is necessary to develop safe and effective vaccines. We designed multi-epitope recombinant proteins A6, A7, and A8 with different three-dimensional structures and compared their immunogenicity in pigs. The results indicated that A8 conferred the greatest protection against FMDV serotype A challenge in pigs, and A8 was selected as the vaccine antigen. We further tested the adjuvant activity of CpG DNA in conjunction with the A8 vaccine, and the results showed significantly increased antigen-specific IFN-y responses in pigs co-administered A8 with CpG compared to those vaccinated with A8 alone. A vaccine potency test showed that the CpG-adjuvanted A8 vaccine contained a 10.81 protective dose 50% (PD50) per dose for pigs, suggesting the potential for this vaccine to be used in emergency vaccination campaigns for the prevention of FMDV serotype A infection in pigs. (C) 2017 Elsevier B.V. All rights reserved.