STIMULATION OF DENOVO METHYLATION FOLLOWING LIMITED PROTEOLYSIS OF MOUSE ASCITES DNA METHYLASE
STIMULATION OF DENOVO METHYLATION FOLLOWING LIMITED PROTEOLYSIS OF MOUSE ASCITES DNA METHYLASE
复制标题
DOI：
10.1016/0014-5793(83)80817-5
复制
发表时间：
1983-01-01
期刊：
影响因子：
3.5
通讯作者：
RINALDI, A
中科院分区：
文献类型：
--
作者：
ADAMS, RLP;BURDON, RH;RINALDI, A
小鼠Krebs II腹水细胞DNA甲基化酶将甲基添加到天然未甲基化DNA（从头活性）的能力受到有限蛋白水解的刺激。这种处理不会改变酶对DNA的亲和力，但反应速率会增加，因此40%或更多的可甲基化位点在4.5小时内被甲基化。活化与酶的大小减少至6.2 S有关。
The ability of mouse Krebs II ascites cell DNA methylase to add methyl groups to native, unmethylated DNA (de novo activity) is stimulated by limited proteolysis. The affinity of the enzyme for DNA is not altered by this treatment but the rate of reaction is increased so that 40% or more of methylatable sites are methylated within 4.5 h. The activation is associated with a decrease in size of the enzyme to 6.2 S.