A near-IR reversible fluorescent probe modulated by selenium for monitoring peroxynitrite and imaging in living cells.
A near-IR reversible fluorescent probe modulated by selenium for monitoring peroxynitrite and imaging in living cells.
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DOI：
10.1021/ja202582x
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发表时间：
2011-07
影响因子：
15
通讯作者：
Fabiao Yu;Peng Li;Guan‐Cheng Li;Guangjiu Zhao;T. Chu;Keli Han
中科院分区：
文献类型：
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作者：
Fabiao Yu;Peng Li;Guan‐Cheng Li;Guangjiu Zhao;T. Chu;Keli Han
我们已经开发了一种近红外可逆荧光探针含有有机硒官能团，可用于高灵敏度和选择性监测过氧亚硝酸盐的氧化和还原事件在生理条件下。该探针有效地避免了生物体系中自发荧光的影响，在水溶液和活细胞中均得到阳性结果。成功地获得了细胞过氧亚硝酸盐的实时图像。
We have developed a near-IR reversible fluorescent probe containing an organoselenium functional group that can be used for the highly sensitive and selective monitoring of peroxynitrite oxidation and reduction events under physiological conditions. The probe effectively avoids the influence of autofluorescence in biological systems and gave positive results when tested in both aqueous solution and living cells. Real-time images of cellular peroxynitrite were successfully acquired.