Sequence analysis of the whole genome of a recombinant Marek’s disease virus strain, GX0101, with a reticuloendotheliosis virus LTR insert
Sequence analysis of the whole genome of a recombinant Marek’s disease virus strain, GX0101, with a reticuloendotheliosis virus LTR insert
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DOI：
10.1007/s00705-013-1671-1
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发表时间：
2013-04
影响因子：
2.7
通讯作者：
S. Su;N. Cui;Ai-jun Sun;Yanpeng Li;Jiabo Ding;Zimeng Chen;P. Zhao;Z. Cui
中科院分区：
文献类型：
--
作者：
S. Su;N. Cui;Ai-jun Sun;Yanpeng Li;Jiabo Ding;Zimeng Chen;P. Zhao;Z. Cui
马立克氏病病毒中国株GX-0101是2001年首次报道的含有网状内皮组织增生病病毒（REV）长末端重复序列（LTR）的重组鸡疱疹病毒2型（GaHV-2）野毒株。我们构建了一个感染性细菌人工染色体（BAC）克隆GX 0101，它在复制和致病性方面与亲本病毒非常相似。利用GX 0101 BAC克隆，对GX 0101的全基因组进行了测序和分析。GX 0101基因组全长178，101 bp，仅在orf 2基因上游267 bp处含有一个REV-LTR插入片段。
Marek’s disease virus Chinese strain GX0101, isolated in 2001, is the first reported recombinant gallid herpesvirus type 2 (GaHV-2) field strain with one reticuloendotheliosis virus (REV) long terminal repeat (LTR) insert. We constructed an infectious bacterial artificial chromosome (BAC) clone of GX0101, which showed characteristics very similar to those of the parental virus in replication and pathogenicity. Using the GX0101 BAC clone, the complete genome of GX0101 was sequenced and analyzed. The length of the GX0101 genome is 178,101 bp, and it contains only one REV-LTR insert at a site 267 bp upstream of thesorf2gene.