Cyto-/genotoxic effects of the ethanol extract of Chan Su, a traditional Chinese medicine, in human cancer cell lines
Cyto-/genotoxic effects of the ethanol extract of Chan Su, a traditional Chinese medicine, in human cancer cell lines
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DOI：
10.1016/j.jep.2014.01.023
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发表时间：
2014-03-14
影响因子：
5.4
通讯作者：
Chung, Hai Won
中科院分区：
文献类型：
--
作者：
Lee, Sunyeong;Lee, Younghyun;Chung, Hai Won
民族药理学相关性：蟾酥是从中华大蟾蜍（Bufo bufo gargarizans Cantor）的皮肤和腮腺毒腺中提取的乙醇提取物，被广泛用作治疗癌症的中药。虽然对蟾酥的抗癌特性进行了研究，但没有关于蟾酥是否对癌细胞具有遗传毒性作用的信息。本研究的目的是研究蟾酥对人乳腺癌（MCF-7细胞），人肺癌（A-549细胞），人T细胞白血病（Jurkat T细胞），和正常人lymphocyte.Materials and Methods的细胞/基因毒性作用：对MCF-7，A-549，Jurkat T细胞，和正常淋巴细胞的活力的影响进行了评估台盼蓝拒染法。碘化丙啶（PI）染色和流式细胞术检测亚G1区DNA含量。结果：蟾酥对MCF-7、A-549和Jurkat T细胞活力有明显的抑制作用，呈剂量依赖性，但对正常人淋巴细胞无明显影响。治疗后癌细胞的凋亡是明显的。Chan Su还诱导遗传毒性的剂量依赖性的方式，如所示的彗星和分裂阻滞微核assays.Conclusions：这些研究结果表明，Chan Su可以诱导细胞凋亡，并发挥遗传毒性作用，MCF-7，A-549，和Jurkat T细胞。(C)2014爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacological relevance: Chan Su, an ethanolic extract from skin and parotid venom glands of the Bufo bufo gargarizans Cantor, is widely used as a traditional Chinese medicine for cancer therapy. Although the anti-cancer properties of Chan Su have been investigated, no information exists regarding whether Chan Su has genotoxic effects in cancer cells. The aim of the present study was to examine the cyto-/genotoxic effect of Chan Su in human breast carcinoma (MCF-7 cells), human lung carcinoma (A-549 cells), human T cell leukemia (Jurkat T cells), and normal human lymphocytes.Materials and methods: Effects on the viability of MCF-7, A-549, Jurkat T cells, and normal lymphocytes were evaluated by Trypan blue exclusion assays. The DNA content in the sub-G1 region was detected by propidium iodide (PI) staining and flow cytometry. The genotoxicity of Chan Su was assessed by single-cell gel electrophoresis (comet assay) and the cytokinesis-block micronucleus assay (CBMN assay).Results: Chan Su significantly inhibited the viability of MCF-7, A-549, and Jurkat T cells dose dependently, but had no effect on normal human lymphocytes. Apoptotic death of the cancer cells was evident after treatment. Chan Su also induced genotoxicity in a dose-dependent manner, as indicated by the comet and cytokinesis-block micronucleus assays.Conclusions: These findings suggest that Chan Su can induce apoptotic death of, and exert genotoxic effects on, MCF-7, A-549, and Jurkat T cells. (C) 2014 Elsevier Ireland Ltd. All rights reserved.