Using multiple quantum coherence to increase the 15N resolution in a three-dimensional TROSY HNCO experiment for accurate PRE and RDC measurements
Using multiple quantum coherence to increase the 15N resolution in a three-dimensional TROSY HNCO experiment for accurate PRE and RDC measurements
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DOI：
10.1016/j.jmr.2009.06.019
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发表时间：
2009-10-01
影响因子：
2.2
通讯作者：
Clore, G. Marius
中科院分区：
文献类型：
--
作者：
Hu, Kaifeng;Doucleff, Michaeleen;Clore, G. Marius
我们提出了一个新版本的3D TROSY HNCO脉冲方案，称为HR-TROSY HNCO，在N-15维的分辨率与2D H-1-N-15 HSQC实验相当。在传统的3D TROSY HNCO中，恒定的时间段（1/2 J（NC），类似于32 ms）严重限制了N-15维度上的最大分辨率。在这里给出的HR-TROSY HNCO实验中，用于N-15和C-13'之间的相干前向和后向传输的两个恒定时间段（每个类似于32 ms）被用于使N-15演化时间加倍。这导致沿N-15维度沿着峰分离的显著增强，使得HR-TROSY HNCO成为用于精确顺磁弛豫增强和残余偶极耦合测量的理想脉冲方案。爱思唯尔公司出版
We present a new version of the 3D TROSY HNCO pulse scheme, referred to as HR-TROSY HNCO, with comparable resolution in the N-15 dimension to a 2D H-1-N-15 HSQC experiment. In the conventional 3D TROSY HNCO, the constant time period (1/2J(NC) similar to 32 ms) severely limits the maximum resolution in the N-15 dimension. In the HR-TROSY HNCO experiment presented here, both constant time periods (similar to 32 ms each) for coherence forward and backward transfer between N-15 and C-13' are utilized to double the N-15 evolution time. This leads to a dramatic enhancement in peak separation along the N-15 dimension, making the HR-TROSY HNCO an ideal pulse scheme for accurate paramagnetic relaxation enhancement and residual dipolar coupling measurements. Published by Elsevier Inc.