VERY LOW DENSITY LIPOPROTEINS IN INTESTINAL LYMPH - ORIGIN, COMPOSITION, AND ROLE IN LIPID TRANSPORT IN FASTING STATE
VERY LOW DENSITY LIPOPROTEINS IN INTESTINAL LYMPH - ORIGIN, COMPOSITION, AND ROLE IN LIPID TRANSPORT IN FASTING STATE
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DOI：
10.1172/jci106174
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发表时间：
1969-01-01
影响因子：
15.9
通讯作者：
ISSELBACHER, KJ
中科院分区：
文献类型：
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作者：
OCKNER, RK;HUGHES, FB;ISSELBACHER, KJ
在禁食的肠系膜淋巴瘘大鼠模型上研究了肠系膜淋巴液脂蛋白中内源性脂质的转运。发现淋巴液中除了乳糜微粒（Sf>400）外，还含有大量另一种密度更大、富含甘油三酯的组分，即极低密度脂蛋白（VLDL），其峰值Sf为102。VLDL与乳糜微粒不仅在漂浮性方面不同，而且在脂质组成百分比和琼脂糖凝胶电泳迁移率方面也不同。发现VLDL组分含有空腹淋巴液中甘油三酯的47%和胆固醇的54%，并且在空腹状态下，是主要的脂蛋白种类，当考来烯胺树脂经尿道内给药时，或胆汁流从肠急性转向时，证明淋巴VLDL中的脂质与乳糜微粒中的脂质一样，来自肠和胆汁。这些数据表明，在肠淋巴中的VLDL不是来自血浆，但肠origin.Because某些性质的淋巴VLDL是类似的血浆VLDL的报告（百分比脂质组成，漂浮系数，并继续进入血浆中的空腹状态），这些馏分之间进行了额外的比较。尽管淋巴VLDL在琼脂糖凝胶中移向α 2区，但在电泳前即刻与去VLDL血清混合时，它们显示出大鼠血清VLDL的α 2迁移率。此外，部分脱脂淋巴液和血清VLDL的免疫电泳比较显示，这些馏分共享共同的主要载脂蛋白，并可能其他。淋巴液和血清甘油三酯的脂肪酸组成，通过气-液色谱法测定，显示，虽然他们一般是相似的，存在的差异，这最有可能反映了存在于血清中的甘油三酯的肝origin.These实验表明肠VLDL的内源性脂质在肠系膜淋巴在禁食状态下的运输的重要性。肠淋巴VLDL和血浆VLDL之间的相似性表明后者可能部分来源于前者。图片
The transport of endogenous lipids in the lipoproteins of mesenteric lymph was studied in fasting rats with mesenteric lymph fistulas. The lymph was found to contain, in addition to chylomicrons (Sf>400), a significant amount of another, more dense, triglyceride-rich fraction, the very low density lipoproteins (VLDL), which showed a peak Sfof 102. The VLDL differed from chylomicrons not only in flotation, but also in per cent lipid composition and electrophoretic mobility in agarose gel. The VLDL fraction was found to contain 47% of the triglyceride and 54% of the cholesterol of fasting lymph and, in the fasting state, was the major lipoprotein species present.When cholestyramine resin was administered intraduodenally, or bile flow was acutely diverted from the intestine, it was demonstrated that the lipids in lymph VLDL, like those in chylomicrons, were derived from the intestine and bile. These data indicate that the VLDL in intestinal lymph are not derived from the plasma but are of intestinal origin.Because certain properties of lymph VLDL were similar to those reported for plasma VLDL (per cent lipid composition, flotation coefficient, and continuing entry into plasma in the fasting state), additional comparisons between these fractions were made. Although lymph VLDL moved to the α2region in agarose gel, when they were mixed with VLDL-free serum immediately before electrophoresis they showed the α2mobility of rat serum VLDL. Furthermore, immunoelectrophoretic comparison of partially delipidated lymph and serum VLDL revealed that these fractions shared in common their major apoprotein, and possibly others as well. The fatty acid composition of lymph and serum triglycerides, as determined by gas-liquid chromatography, revealed that although they were generally similar, differences existed which most likely reflected the presence in serum of triglycerides of hepatic origin.These experiments demonstrate the importance of intestinal VLDL in the transport of endogenous lipids in mesenteric lymph in the fasting state. The similarities between intestinal lymph VLDL and plasma VLDL suggest that the latter may be derived in part from the former.Images