Fundulus heteroclitus gonadotropins. 3. Cloning and sequencing of gonadotropic hormone (GTH) I and II β-subunits using the polymerase chain reaction
Fundulus heteroclitus gonadotropins. 3. Cloning and sequencing of gonadotropic hormone (GTH) I and II β-subunits using the polymerase chain reaction
复制标题
眼底异斜促性腺激素 3. 使用聚合酶链式反应对促性腺激素 (GTH) I 和 II β 亚基进行克隆和测序。
DOI：
10.1016/0303-7207(92)90132-p
复制
发表时间：
1992
影响因子：
4.1
通讯作者：
R. Wallace
中科院分区：
文献类型：
--
作者：
Y.;B. Rupnow;D. Price;R. Greenberg;R. Wallace
从异阴蒂垂体底提取mRNA，构建λ gt 22 A cDNA文库。根据硬骨鱼促性腺激素（GTH）β亚基的保守序列，设计合成了一系列寡核苷酸序列，并以此为引物进行PCR扩增。将适当长度的PCR产物亚克隆并测序。8个克隆最终被鉴定为编码F的两个不同β亚基的cDNA。heteroclitus，GTH I和GTH II。通过与已知GTH序列的比较，发现GTH Ⅰ和GTH Ⅱ分别含有19 - 21个氨基酸的信号肽序列和95 - 115个氨基酸的成熟β亚基。当与糖蛋白家族的其他成员比对时，两个β亚基具有良好保守的半胱氨酸位置。两种F. heteroclitusGTH提供了明确的证据，证明在该物种中至少有两种不同形式的垂体GTH，类似于经典的促黄体激素-促卵泡激素家族。
mRNA was isolated fromFundulus heteroclituspituitaries and used to construct a cDNA library in λgt22A. A series of synthetic oligonucleotides, based on conserved regions of teleost gonadotropic hormone (GTH) β-subunits, were constructed and used as primers in the polymerase chain reaction (PCR) to amplify GTH cDNAs. Appropriate length PCR products were subcloned and sequenced. Eight clones were eventually identified as cDNAs encoding two distinct β-subunits ofF. heteroclitus, GTH I and GTH II. By comparison with known GTH sequences, putative signal sequences of 19 end 21 amino acids and mature β-subunits of 95 and 115 amino acids were found for GTH I and GTH II, respectively. Both β-subunits had well conserved cysteine positions when aligned with other members of the glycoprotein family. The elucidation of the complete nucleotide sequences of two types ofF. heteroclitusGTH provides definitive proof that in this species there are at least two distinct forms of pituitary GTH analogous to the classical luteinizing hormone-follicle stimulating hormone family.
二硫键形成在人绒毛膜促性腺激素β亚基折叠成αβ二聚体组装能力形式中的作用。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Beebe,JS;Mountjoy,K;Krzesicki,RF;Perini,F;Ruddon,RW
通讯作者：
Ruddon,RW
牛促甲状腺素 β 亚基的克隆 cDNA 序列预测蛋白质含有 NH2 和 COOH 末端延伸。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Maurer,RA;Croyle,ML;Donelson,JE
通讯作者：
Donelson,JE
对几种牛促生长素 β 亚基 cDNA 的分析揭示了核苷酸序列的异质性。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Maurer,RA
通讯作者：
Maurer,RA