Phylogenetic characterization of 'Candidatus Helicobacter bovis', a new gastric helicobacter in cattle
Phylogenetic characterization of 'Candidatus Helicobacter bovis', a new gastric helicobacter in cattle
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DOI：
10.1099/00207713-49-4-1707
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发表时间：
1999-10-01
期刊：
影响因子：
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通讯作者：
Vandamme, P
中科院分区：
文献类型：
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作者：
De Groote, D;van Doorn, LJ;Vandamme, P
近年来，在犊牛和成年牛的皱胃幽门部发现了类螺旋杆菌。迄今为止，这些螺旋细菌的培养还没有成功。在本研究中，比较16S rDNA序列分析被用来确定这些细菌的分类位置。从不同的比利时和荷兰农场成年牛的皱胃活检样品。在所有样品中的螺旋杆菌样生物的存在下，证明了生物化学，免疫组织化学和电子显微镜数据。通过PCR扩增细菌16S rDNA，并通过直接或间接序列分析确定序列。成对比较显示所有序列的同源性超过99%。系统发育分析将对应于参考序列R2XA的生物体置于螺杆菌属内。设计了一种诊断PCR方法，区分所有的牛16S rDNA序列从螺杆菌和Wolinella物种。胆汁螺杆菌（Helicobacter bilis）（92.8%）与其最近的有效命名邻居的相似性较低，表明该新分类群确实是一种新的螺杆菌物种。一个原位杂交程序相关联的牛序列的皱胃中的螺旋杆菌样生物体。建议将这种来自牛的新皱胃螺旋杆菌命名为“牛幽门螺杆菌”。
Recently helicobacter-like organisms have been reported in the pyloric part of the abomasum of calves and adult cattle. Cultivation of these spiral bacteria has not been successful to date. In the present study, comparative 16S rDNA sequence analysis was used to determine the taxonomic position of these bacteria. Seven abomasal biopsies of adult cattle were sampled from different Belgian and Dutch farms. In all samples the presence of helicobacter-like organisms was demonstrated by biochemical, immunohistochemical and electron microscopical data. Bacterial 16S rDNA was amplified by PCR and sequences were determined either by direct or indirect sequence analysis. Pairwise comparisons revealed all sequences to be more than 99% homologous. Phylogenetic analysis placed the organism, corresponding to the reference sequence R2XA, within the genus Helicobacter. A diagnostic PCR assay was designed, differentiating all of the bovine 16S rDNA sequences from Helicobacter and Wolinella species. The low similarity level towards Helicobacter bilis (92.8%), its closest validly named neighbour, indicates that this novel taxon is indeed a novel Helicobacter species. An in situ hybridization procedure associated the bovine sequences to the helicobacter-like organisms in the abomasum. The name 'Candidatus Helicobacter bovis' is proposed for this new abomasal helicobacter from cattle.