Bacteriocin production and gene sequencing analysis from vaginal Lactobacillus strains
Bacteriocin production and gene sequencing analysis from vaginal Lactobacillus strains
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DOI：
10.1007/s00203-014-1003-1
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发表时间：
2014-09-01
影响因子：
2.8
通讯作者：
Torriani, Sandra
中科院分区：
文献类型：
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作者：
Stoyancheva, Galina;Marzotto, Marta;Torriani, Sandra
人类阴道是一个复杂而动态的生态系统，含有丰富的微生物。在育龄妇女中，该系统由乳杆菌属（Lactobacillus spp.）在本工作中，17个新分离的阴道菌株进行了鉴定16S rDNA测序，并调查其抗菌性能。12个分离的乳杆菌菌株显示出对一种或多种微生物的活性。其中六种和五种分别产生抑制两种不同克雷伯氏菌菌株和金黄色葡萄球菌生长的物质。两种乳酸杆菌菌株对大肠杆菌菌株有活性，一种分离株对粪肠球菌菌株有活性，另一种乳酸杆菌菌株对近平滑念珠菌菌株显示出抗微生物活性。另外研究了活性化合物的性质，并证明了细菌素样物质的存在。对其中3株新分离的细菌素产生相关基因进行了鉴定和测序。第一次描述了卷曲乳杆菌基因组中gassericin A操纵子的存在。抗微生物活性的存在有助于它们在进一步研究后作为潜在益生菌菌株的可能用途。
The human vagina is a complex and dynamic ecosystem containing an abundance of microorganisms. In women of childbearing age, this system is dominated by Lactobacillus spp. In the present work, seventeen newly isolated vaginal strains were identified by 16S rDNA sequencing and were investigated for their antimicrobial properties. Twelve of the isolated Lactobacillus strains showed activity against one or more microorganisms. Six and five of them produced substances that inhibited the growth of two different Klebsiella strains and Staphylococcus aureus, respectively. Two lactobacilli strains were active against an Escherichia coli strain, one isolate was active against an Enterococus faecalis strain and another lactobacilli strain showed antimicrobial activity against a Candida parapsilosis strain. The nature of the active compounds was additionally studied, and the presence of bacteriocin-like substances was proved. The genes related to the bacteriocin production in three of the newly isolated strains were identified and sequenced. The presence of gassericin A operon in the genome of the species Lactobacillus crispatus was described for the first time. The presence of antimicrobial activity contributes to their possible use as potential probiotic strains after further research.