Heat-treated membranes with bioelectricity promote bone regeneration
Heat-treated membranes with bioelectricity promote bone regeneration
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DOI：
10.1080/09205063.2013.849903
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发表时间：
2014-01
期刊：
影响因子：
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通讯作者：
Y. Qu;Yanying Wang;Xiang-li Kong;Jidong Li;Y. Zuo;Q. Zou;P. Gong;Y. Man
中科院分区：
文献类型：
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作者：
Y. Qu;Yanying Wang;Xiang-li Kong;Jidong Li;Y. Zuo;Q. Zou;P. Gong;Y. Man
屏障膜保持一个隐蔽的空间，以防止结缔组织向内生长，并引导新骨生长到所需部位;然而，它们不刺激或诱导骨再生。为了提高膜的骨生物活性，我们采用栅控恒压电晕充电法制备了具有生物电的壳聚糖驻极体膜。经过热处理的驻极体膜（HT驻极体膜）比未经热处理的驻极体膜（RT驻极体膜）表现出更好的上级驻极体电荷储存稳定性。人骨髓基质细胞（hBMSCs）在HT驻极体膜上生长良好。此外，hBMSCs成骨细胞分化的HT驻极体膜，骨钙素和骨桥蛋白的表达所证明的免疫细胞化学，定量RT-PCR和蛋白质印迹分析评估。兔颅骨缺损模型表明，HT驻极体膜诱导骨再生显着增强RT驻极体膜相比。植入4周后，在缺损的周围和中心均发现新骨形成。这些结果表明，壳聚糖驻极体膜具有成骨潜能，可作为一种新型的屏障膜。
The barrier membranes maintain a secluded space to prevent the ingrowth of connective tissue and direct the growth of new bone into a desired site; however, they do not stimulate or induce bone regeneration. To enhance the bone bioactivities of membranes, we developed chitosan electret membranes with bioelectricity by grid-controlled constant voltage corona charging. The electret membranes charged with heat treatment (HT electret membranes) exhibited superior electret charge storage stability than the ones charged without heat treatment (RT electret membranes). Human bone marrow stromal cells (hBMSCs) demonstrated better growth on HT electrets membrane. Moreover, hBMSCs osteoblastic differentiation was enhanced on HT electret membranes, as evidenced by osteocalcin and osteopontin expression as assessed by immunocytochemistry, quantitative RT-PCR and western blot analysis. The rabbit calvarial defect model demonstrated that HT electret membranes induced a significantly enhanced bone regeneration compared with RT electret membranes. New bone formation was found at both the periphery and in the center of the defects four weeks after implantation. These results indicated that the chitosan electret membrane has osteogenic potential and could be applied as a novel barrier membrane.