FLOW CYTOMETRIC EVALUATION OF ERYTHROCYTE RESPONSE TO OXIDANT STRESS
FLOW CYTOMETRIC EVALUATION OF ERYTHROCYTE RESPONSE TO OXIDANT STRESS
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DOI：
10.1002/cyto.990200105
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发表时间：
1995-05-01
期刊：
影响因子：
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通讯作者：
FAKEIR, A
中科院分区：
文献类型：
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作者：
HAMMOUDA, AERMA;FAKEIR, A
红细胞对氧化剂挑战的反应已被用作细胞膜对氧化应激耐受性的指标。本研究描述了一种简单的技术，用于评估红细胞对过氧化氢氧化应激的反应，使用流式细胞术作为测定方法。染色的细胞显示出可检测的荧光增加超过基础水平。荧光的这种增加是浓度和时间依赖性的。使用0.1 mM过氧化氢和2 h孵育对试验进行标准化。标准化试验显示，与挑战的成人红细胞相比，挑战的脐带红细胞的荧光更高。我们建议进一步使用该试验检查其他红细胞群。(C)1995年Wiley-Liss，Inc.
Erythrocyte response to oxidant challenge has been used as an indicator of cell membrane tolerance to oxidant stress. This study describes a simple technique for evaluation of erythrocyte response to hydrogen peroxide oxidant stress using now cytometry as an assay method. Challenged cells showed a detectable increase in fluorescence over the basal level. This increase in fluorescence was both concentration and time dependent. The test was standardized using 0.1 mM hydrogen peroxide and 2 h incubation. The standardized test showed a higher fluorescence of challenged umbilical cord erythrocytes compared to challenged adult red blood cells. We recommend further use of this test for examining other populations of red blood cells. (C) 1995 Wiley-Liss, Inc.