Characterization of monoclonal antibodies generated to the cornified envelope of human cultured keratinocytes.
Characterization of monoclonal antibodies generated to the cornified envelope of human cultured keratinocytes.
复制标题
针对人类培养的角质形成细胞的角质化包膜产生的单克隆抗体的表征。
DOI：
10.1111/1523-1747.ep12460759
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Phillips,SB
中科院分区：
文献类型：
--
作者：
Baden,HP;Kubilus,JK;Phillips,SB
角质形成细胞的角质层包膜是在角质层基底处的质膜下形成的不溶性结构。它是由膜相关转氨酶交联前体蛋白质制成的。我们已经制备了培养的人角质形成细胞的皮质包膜的单克隆抗体，并使用这些蛋白质印迹法来识别前体蛋白。我们已经发现了一些前体，包括外皮蛋白和一个195 kD的膜相关蛋白，这是以前报道过的。这些前体的抗体，除了一个外皮蛋白，与其他哺乳动物物种的表皮反应，这表明在至少一些信封组件的结构保护。
The cornified envelope of keratinocytes is an insoluble structure formed beneath the plasma membrane at the base of the stratum corneum. It is made by cross-linking precursor proteins by a membrane-associated transglutaminase. We have prepared monoclonal antibodies to the cornified envelope of cultured human keratinocytes and used these to identify precursor proteins using Western blotting. We have uncovered a number of precursors including involucrin and a 195 kD membrane-associated protein, which had previously been reported. Antibodies to these precursors, with the exception of the one to involucrin, reacted with the epidermis of other mammalian species, suggesting structural conservation in at least some envelope components.