Electrochemical detection at low temperature for a specific nucleobase of target nucleic acids by an abasic site-containing DNA binding ligand
Electrochemical detection at low temperature for a specific nucleobase of target nucleic acids by an abasic site-containing DNA binding ligand
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DOI：
10.1016/j.elecom.2005.12.021
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发表时间：
2006-03-01
影响因子：
5.4
通讯作者：
Teramae, N
中科院分区：
文献类型：
--
作者：
Huang, W;Morita, K;Teramae, N
演示了基于硫醇化 DNA 双链体中无碱基位点（AP 位点）制造的单核苷酸多态性分析。 AP位点允许电化学指示剂与目标核苷酸结合并伴随电化学信号传导并检测目标核酸的特定核碱基。含有硫醇化 AP 位点的探针 DNA 的混合自组装单层与靶 DNA 杂交，从而将 AP 位点引向靶核碱基。这样，为电化学指示剂提供了疏水性微环境，以基于电化学响应的差异来识别DNA双链体上的特定核碱基。发现电化学反应在低温下增强。含 AP 位点的 DNA 双链体中温度诱导的核黄素电化学响应增强，用于基于电化学方法专门检测胸苷。 (c) 2006 Elsevier B.V. 保留所有权利。
Single-nucleotide polymorphism analysis based on fabrication of the abasic site (AP site) in the thiolated DNA duplexes is demonstrated. The AP site allows an electrochemical indicator to bind to target nucleotides accompanied by electrochemical signaling and to detect a specific nucleobase of a target nucleic acid. A mixed self-assembled monolayer of thiolated AP site-containing probe DNA is hybridized with the target DNA so as to direct the AP site toward the target nucleobase. In this way, hydrophobic microenvironments are provided for the electrochemical indicator to recognize the specific nucleobase on the DNA duplex based on the difference in the electrochemical response. The electrochemical response is found to be strengthened at low temperature. The enhanced electrochemical response of riboflavin induced by temperature in an AP site-containing DNA duplex is used to detect thymidine specifically based on an electrochemical approach. (c) 2006 Elsevier B.V. All rights reserved.