The genomic organization of type I keratin genes in mice.
The genomic organization of type I keratin genes in mice.
复制标题
小鼠 I 型角蛋白基因的基因组组织。
作者：
H. Sato;T. Koide;T. Sagai;S. Ishiguro;M. Tamai;N. Saitou;T. Shiroishi
我们以人KRT14为探针，从C57BL/10J小鼠背部和腹部皮肤的Poly(A)+RNA中筛选出两个新的角蛋白cDNAs。由于其与人角蛋白17(Krt1-17)有很高的同源性，其中一个全长基因很可能是鼠角蛋白17(Krt1-17)。另一个是一个新的I型角蛋白基因的全长cdna，命名为Krt1-c29。这两个角蛋白基因被定位在11号染色体的远端，小鼠角蛋白基因复合体-1(Krt1)位于该区域。为了阐明Krt1在小鼠中的基因组结构，我们对Krt1进行了遗传和物理分析。利用亚种间回交进行的大规模连锁分析表明，Krt1有两个主要的类群，一个包含Krt1-c29、Krt1-10和Krt1-12，另一个包含Krt1-14、-15、-17和-19。两个酵母人工染色体克隆的截断实验表明，Krt1的基因序列为着丝粒-Krt1-c29-Krt1-10-Krt1-12-Krt1-13-Krt1-15-Krt1-19-Krt1-14-Krt1-17-端粒。最后，我们分析了属于Krt1复合体的基因之间的序列差异。结果清楚地表明，根据系统发育关系，基因可分为两大类。每一组都由本研究中的遗传和物理分析所证明的各自的基因簇组成，这表明Krt1复合体的物理组织反映了该复合体基因复制的进化过程。
We isolated two new keratin cDNAs by screening a cDNA library constructed from poly(A)+ RNA of the dorsal and abdominal skin of C57BL/10J mice with a probe of human KRT14. Due to its high sequence homology to human keratin 17 cDNA, one full-length cDNA is most likely to be mouse keratin 17 (Krt1-17) cDNA. The other is the putative full-length cDNA of a novel type I keratin gene, designated Krt1-c29. These two keratin genes were mapped to the distal portion of Chromosome 11, where the mouse keratin gene complex-1 (Krt1) is localized. To elucidate the genomic organization of Krt1 in mice, we carried out genetic and physical analyses of Krt1. A large-scale linkage analysis using intersubspecific backcrosses suggested that there are two major clusters in Krt1, one containing Krt1-c29, Krt1-10, and Krt1-12 and the other containing Krt1-14, -15, -17, and -19. Truncation experiments with two yeast artificial chromosome clones containing the two clusters above have revealed that the gene order of Krt1 is centromere-Krt1-c29-Krt1-10-Krt1-12-Krt1-13-K rt1-15-Krt1-19-Krt1-14-K rt1-17-telomere. Finally, we analyzed sequence divergence between the genes belonging to the Krt1 complex. The results clearly indicated that genes are classified into two major groups with respect to phylogenetic relationship. Each group consists of the respective gene cluster demonstrated by genetic and physical analyses in this study, suggesting that the physical organization of the Krt1 complex reflects the evolutionary process of gene duplication of this complex.
影响因子：
4.4
作者：
W. Kao;Chia-Yang Liu;R. Converse;Atsushi Shiraishi;C. Kao;Masamichi Ishizaki;Thomas Doetschman;John J. Duffy
通讯作者：
W. Kao;Chia-Yang Liu;R. Converse;Atsushi Shiraishi;C. Kao;Masamichi Ishizaki;Thomas Doetschman;John J. Duffy
基于赖氨酸2的酵母人工染色体断裂载体的创建。
DOI：
10.1016/1050-3862(92)90003-n
发表时间：
1992
期刊：
Genetic analysis, techniques and applications
影响因子：
--
作者：
Lewis,BC;Shah,NP;Braun,BS;Denny,CT
通讯作者：
Denny,CT
I 型角蛋白基因家族和同源盒-2 基因复合体与小鼠 11 号染色体上的 rex 基因座紧密相连。
影响因子：
4.4
作者：
Nadeau,JH;Berger,FG;Cox,DR;Crosby,JL;Davisson,MT;Ferrara,D;Fuchs,E;Hart,C;Hunihan,L;Lalley,PA
通讯作者：
Lalley,PA