RELEASE OF SECRETORY PRODUCTS DURING TRANSIENT VESICLE FUSION
RELEASE OF SECRETORY PRODUCTS DURING TRANSIENT VESICLE FUSION
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DOI：
10.1038/363554a0
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发表时间：
1993-06-10
期刊：
影响因子：
64.8
通讯作者：
FERNANDEZ, JM
中科院分区：
文献类型：
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作者：
DETOLEDO, GA;FERNANDEZCHACON, R;FERNANDEZ, JM
PATCH-CLAMP实验表明，分泌颗粒与质膜的融合并不总是以全有或全无的方式发生，而是可以以波动的方式缓慢发展，或者可以是短暂的1 -5。这些观测结果表明，在这种不完全融合事件中可以检测到释放。为了验证这一假设，我们结合膜片钳测量的活动，单个胞吐融合孔在米色小鼠肥大细胞的电化学检测5-羟色胺释放过程中的胞吐事件。我们在这里报告，融合孔开放有一个小的5-羟色胺的释放，这是成正比的孔电导。我们还表明，在瞬时聚变事件期间会发生显着的释放。这些结果表明，据我们所知，第一次，从分泌囊泡，没有经历完全融合的神经递质的释放。
PATCH-CLAMP experiments have shown that fusion of secretory granules with the plasma membrane does not always occur as an all-or-none event, but can develop slowly in a fluctuating manner or can be transient1-5. These observations suggested that release could be detected during such incomplete fusion events. To test this hypothesis we have combined patch-clamp measurements of the activity of single exocytotic fusion pores in beige mouse mast cells with the electrochemical detection of serotonin released during the exocytotic events. We report here that on fusion pore opening there is a small release of serotonin which is directly proportional to the pore conductance. We also show that a significant release occurs during transient fusion events. These results demonstrate, to our knowledge for the first time, release of a neurotransmitter from a secretory vesicle that did not undergo complete fusion.