Two-photon excited fluorescence of intrinsic fluorophores enables label-free assessment of adipose tissue function.
Two-photon excited fluorescence of intrinsic fluorophores enables label-free assessment of adipose tissue function.
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DOI：
10.1038/srep31012
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发表时间：
2016-08-05
影响因子：
4.6
通讯作者：
Georgakoudi I
中科院分区：
文献类型：
--
作者：
Alonzo CA;Karaliota S;Pouli D;Liu Z;Karalis KP;Georgakoudi I
目前用于评估脂肪组织功能的方法是破坏性的或具有低空间分辨率。这些限制了我们评估健康或患病受试者或治疗期间发生的动态变化和异质反应的能力。在这里，我们证明了内在的双光子激发荧光能够以亚细胞分辨率对脂肪细胞代谢进行功能成像。来自细胞内代谢辅因子和脂滴的稳态和时间分辨荧光可以区分切除的白色、棕色和冷诱导的米色脂肪的功能状态。当在体内评估白色和棕色脂肪时，识别出类似的光学变化。因此，这些研究确立了非侵入性、高分辨率、内源性对比度、双光子成像的潜力，以识别不同的脂肪组织类型，监测其功能状态，并表征诱导反应的异质性。
Current methods for evaluating adipose tissue function are destructive or have low spatial resolution. These limit our ability to assess dynamic changes and heterogeneous responses that occur in healthy or diseased subjects, or during treatment. Here, we demonstrate that intrinsic two-photon excited fluorescence enables functional imaging of adipocyte metabolism with subcellular resolution. Steady-state and time-resolved fluorescence from intracellular metabolic co-factors and lipid droplets can distinguish the functional states of excised white, brown, and cold-induced beige fat. Similar optical changes are identified when white and brown fat are assessed in vivo. Therefore, these studies establish the potential of non-invasive, high resolution, endogenous contrast, two-photon imaging to identify distinct adipose tissue types, monitor their functional state, and characterize heterogeneity of induced responses.