A study of the permeability barrier in epidermis and oral epithelium using horseradish peroxidase as a tracer in vitro
A study of the permeability barrier in epidermis and oral epithelium using horseradish peroxidase as a tracer in vitro
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以辣根过氧化物酶为示踪剂的体外表皮和口腔上皮渗透屏障研究
DOI：
10.1111/j.1365-2133.1976.tb00814.x
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发表时间：
1976
影响因子：
10.3
通讯作者：
R. Hopps
中科院分区：
文献类型：
--
作者：
C. Squier;R. Hopps
将皮肤、角化和非角化口腔粘膜的小活检组织与辣根过氧化物酶一起孵育，固定并反应，以在光学和电子显微镜水平上证明过氧化物酶活性。在所有组织中，过氧化物酶通过上皮细胞间隙延伸至角化组织的角质层或非角化组织的浅表层，但未进入角质层。该分布对应于在体内使用示踪剂的研究中观察到的分布，其中在上层细胞中已经证明存在细胞间渗透性屏障。这表明，该方法是一种有用的方法，调查正常和病理人体上皮组织的渗透性。
Small biopsies of skin, and of keratinized and non‐keratinized oral mucosa were incubated with horseradish peroxidase, fixed and reacted to demonstrate peroxidase activity at the light and electron microscope level. In all tissues peroxidase extended through the intercellular spaces of the epithelium up to, but not into, the stratum corneum of keratinized, or the superficial layer of non‐keratinized, tissue. This distribution corresponds to that seen in studies using the tracer in vivo where the presence of an intercellular permeability barrier has been demonstrated in the upper cell layers. It is suggested that the method is a useful way of investigating the permeability of normal and pathological human epithelial tissues.