A RCA-based assay for analyzing individual strand break in DNA heteroduplex cleavage by restriction endonucleases.
A RCA-based assay for analyzing individual strand break in DNA heteroduplex cleavage by restriction endonucleases.
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DOI：
10.1039/c4cc05314a
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发表时间：
2014-09
影响因子：
4.9
通讯作者：
Guojie Zhao;Tianyu Hu;Jun Li;Hua Wei;H. Shang;Y. Guan
中科院分区：
文献类型：
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作者：
Guojie Zhao;Tianyu Hu;Jun Li;Hua Wei;H. Shang;Y. Guan
我们建立了一种基于滚环扩增的快速、高通量的方法，通过限制性内切酶来区分DNA双链的单链切割。作为例证，我们用EcoRI分析了Nb.BbvCI的划痕活性和LNA修饰的DNA的不均匀切割。这种分析方法具有分析蛋白质-DNA相互作用的潜力。
We have developed a rapid and high-throughput assay based on rolling circle amplification, to distinguish individual strand cleavage of DNA duplexes by restriction endonucleases. As an illustration, we analyzed nicking activity of Nb.BbvCI and uneven cleavage of LNA modified DNA by EcoRI. This assay has potential for analyzing protein-DNA interactions.