PINCH-2 expression in cancers involving serosal effusions using quantitative PCR.
PINCH-2 expression in cancers involving serosal effusions using quantitative PCR.
复制标题
使用定量PCR涉及浆膜积液的癌症中的捏合-2表达。
DOI：
10.1111/j.1365-2303.2010.00757.x
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发表时间：
2011-02
期刊：
影响因子：
--
通讯作者：
Davidson B
中科院分区：
文献类型：
--
作者：
Yuan Y;Dong HP;Nymoen DA;Nesland JM;Wu C;Davidson B
先前在Affyssin阵列分析中显示，与卵巢/腹膜浆液性癌相比，PINCH-2基因在恶性间皮瘤中过表达。本研究的目的是在mRNA和蛋白质水平验证这一发现。使用定量PCR测定积液（n = 91; 71个卵巢癌和10个乳腺癌，10个恶性间皮瘤）的PINCH-2 mRNA表达。使用流式细胞术分析37例渗出液中的PINCH-2蛋白表达。定量PCR分析显示间皮瘤中PINCH-2 mRNA水平显著高于癌（P = 0.004）。<10拷贝的值仅见于癌性渗出液（25.4%的卵巢癌和50%的乳腺癌）。然而，通过流式细胞术检测的PINCH-2蛋白表达在三种癌症类型之间没有显著差异。PINCH-2水平与患者生存率或先前研究的与卵巢癌粘附、转移和凋亡抑制相关的分子表达之间没有相关性。我们的数据表明，PINCH-2 mRNA在恶性间皮瘤中过表达，与涉及浆膜腔的癌相比，该基因的低水平反对间皮瘤的诊断。PINCH-2蛋白在所有三种研究的癌症中的频繁表达表明该分子在渗出液中的癌细胞生物学中的作用，值得进一步研究。
The PINCH-2 gene was previously shown to be overexpressed in malignant mesothelioma compared with ovarian/peritoneal serous carcinoma in Affymetrix array analysis. The objective of the present study was to validate this finding at the mRNA and protein level. Effusions (n = 91; 71 ovarian and 10 breast carcinomas, 10 malignant mesotheliomas) were assayed for PINCH-2 mRNA expression using quantitative PCR. PINCH-2 protein expression was analysed in 37 effusions using flow cytometry. Quantitative PCR analysis showed significantly higher PINCH-2 mRNA levels in mesotheliomas compared with carcinomas (P = 0.004). Values of <10 copies were found exclusively in carcinoma effusions (25.4% of ovarian and 50% of breast carcinomas). However, PINCH-2 protein expression by flow cytometry did not differ significantly between the three cancer types. No association was observed between PINCH-2 levels and patient survival or expression of previously-studied molecules related to adhesion, metastasis and apoptosis inhibition in ovarian carcinoma. Our data suggest that PINCH-2 mRNA is overexpressed in malignant mesothelioma compared with carcinomas involving serosal cavities, and that low levels of this gene argue against the diagnosis of mesothelioma. The frequent PINCH-2 protein expression in all three studied cancers suggests a role for this molecule in cancer cell biology in effusions and merits further research.
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影响因子：
4
作者：
Givant-Horwitz, V;Davidson, B;Reich, R
通讯作者：
Reich, R
影响因子：
11.4
作者：
Beillard, E;Pallisgaard, N;Gabert, J
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Gabert, J
影响因子：
4.6
作者：
AuBuchon, J. P.;de Wildt-Eggen, J.;Dumont, L. J.
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Dumont, L. J.
影响因子：
6.2
作者：
Davidson, Ben;Skredel, Martina;Florenes, Vivi Ann
通讯作者：
Florenes, Vivi Ann
影响因子：
6.6
作者：
Dougherty, Gerard W.;Jose, Cynthia;Cutler, Mary Lou
通讯作者：
Cutler, Mary Lou