Effect of porous structure of macroporous polymer supports on resolution in high-performance membrane chromatography of proteins.
Effect of porous structure of macroporous polymer supports on resolution in high-performance membrane chromatography of proteins.
复制标题
大孔聚合物载体的多孔结构对蛋白质高效膜色谱分离度的影响。
DOI：
10.1016/s0021-9673(97)00873-x
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Svec,F
中科院分区：
文献类型：
--
作者：
Tennikov,MB;Gazdina,NV;Tennikova,TB;Svec,F
研究了2 mm厚二乙胺功能化单片聚甲基丙烯酸酯圆盘的多孔结构对其离子交换模式色谱行为的影响。孔径小的圆盘表现不佳，因为它们具有高背压和大量的峰展宽。孔隙大于1 000 nm的圆盘也不能提供很好的分离，因为一些蛋白质分子穿过孔隙到达壁上进行吸附/解吸过程所需的时间可能比它们在基质中的停留时间更长。最佳孔径以700 nm为中心。即使在非常陡峭的梯度和高流速下，这些圆盘也能实现出色的分离，从而大大缩短了分离时间。
The effect of porous structures of 2-mm thick diethylamine functionalized monolithic polymethacrylate discs on their chromatographic behavior in ion-exchange mode has been studied. Discs with small pores did not perform well because they exhibited high back pressure and substantial peak broadening. Discs characterized with pores larger than 1 000 nm did not provide good separations either because the time required for some protein molecules to traverse the length across the pore to reach the wall for adsorption/desorption process that is essential for the separation may be longer than their residence time within the matrix. Optimum pore size is centered at about 700 nm. Excellent separations have been achieved with these discs even at very steep gradients and high flow-rates which allow to shorten the separation times substantially.