Hollow hydroxyapatite microspheres/chitosan composite as a sustained delivery vehicle for rhBMP-2 in the treatment of bone defects
Hollow hydroxyapatite microspheres/chitosan composite as a sustained delivery vehicle for rhBMP-2 in the treatment of bone defects
复制标题
中空羟基磷灰石微球/壳聚糖复合材料作为 rhBMP-2 持续递送载体治疗骨缺损。
DOI：
10.1007/s10856-014-5336-8
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Wang DP
中科院分区：
文献类型：
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作者：
Yao AH;Li XD;Xiong L;Zeng JH;Xu J.;Wang DP
制备了中空羟基磷灰石（HA）和壳聚糖（CS）复合支架（hHA/CS），作为重组人骨形态发生蛋白2（rhBMP 2）的载体。然后研究复合支架释放的rhBMP 2的体内外生物学活性。首先将rhBMP-2负载到中空HA微球中，然后将负载rhBMP-2的HA微球进一步掺入到壳聚糖基质中。壳聚糖不仅可以将HA微球结合在一起并保持在植入部位，还可以有效地改变rhBMP-2的释放行为。体外释放实验和生物活性分析表明，rhBMP 2能够以生物活性形式从复合支架上负载和释放。此外，与CS支架相比，复合支架显著降低了rhBMP 2的初始突释，从而提供了延长的时间段（长达60天）。在兔桡骨缺损模型中评价负载rhBMP 2的复合支架的体内骨再生潜力。结果显示，rhBMP 2负载hHA/CS组的新骨形成率高于阴性对照组和rhBMP 2负载CS组。这些观察结果表明，hHA/CS复合支架作为骨再生和修复中生长因子的递送载体是有效和可行的。
Composite scaffold comprised of hollow hydroxyapatite (HA) and chitosan (designated hHA/CS) was prepared as a delivery vehicle for recombinating human bone morphogenetic protein-2 (rhBMP-2). The in vitro and in vivo biological activities of rhBMP2 released from the composite scaffold were then investigated. The rhBMP-2 was firstly loaded into the hollow HA microspheres, and then the rhBMP2-loaded HA microspheres were further incorporated into the chitosan matrix. The chitosan not only served to bind the HA microspheres together and kept them at the implant site, but also effectively modified the release behavior of rhBMP-2. The in vitro release and bioactivity analysis confirmed that the rhBMP2 could be loaded and released from the composite scaffolds in bioactive form. In addition, the composite scaffolds significantly reduced the initial burst release of rhBMP2, and thus providing prolonged period of time (as long as 60 days) compared with CS scaffolds. In vivo bone regenerative potential of the rhBMP2-loaded composite scaffolds was evaluated in a rabbit radius defect model. The results revealed that the rate of new bone formation in the rhBMP2-loaded hHA/CS group was higher than that in both negative control and rhBMP2-loaded CS group. These observations suggest that the hHA/CS composite scaffold would be effective and feasible as a delivery vehicle for growth factors in bone regeneration and repair.