DISTRIBUTION OF ERYTHROMYCIN ESTERASE AND RIBOSOMAL-RNA METHYLASE GENES IN MEMBERS OF THE FAMILY ENTEROBACTERIACEAE HIGHLY RESISTANT TO ERYTHROMYCIN
DISTRIBUTION OF ERYTHROMYCIN ESTERASE AND RIBOSOMAL-RNA METHYLASE GENES IN MEMBERS OF THE FAMILY ENTEROBACTERIACEAE HIGHLY RESISTANT TO ERYTHROMYCIN
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DOI：
10.1128/aac.31.3.404
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发表时间：
1987-03-01
影响因子：
4.9
通讯作者：
COURVALIN, P
中科院分区：
文献类型：
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作者：
ARTHUR, M;ANDREMONT, A;COURVALIN, P
通过菌落杂交研究了112株对红霉素高度耐药的肠杆菌科成员中与ereA、ereB和ermAM相关的核苷酸序列的分布。ereA和ereB基因编码红霉素酯酶I型和II型，分别检测到菌株灭活14元大环内酯红霉素和竹桃霉素。由于所有52株通过灭活耐这些抗生素的菌株均被ereA（n = 23）、ereB（n = 23）或两种探针（n = 6）检测到，因此只有两类基因导致了这种耐药表型。在21株大肠埃希菌和2株克雷伯菌中检测到编码链球菌rRNA甲基化酶的ermAM基因。大环内酯类、林可酰胺类和链阳性菌素（MLS）抗生素的MIC测定表明，与ermAM杂交和所谓的MLS耐药表型之间存在相关性。存在11株对MLS抗生素共耐药的菌株，这些菌株未对CRAMAM探针进行超敏处理，这表明与革兰氏阳性菌一样，肠杆菌科成员的MLS耐药性涉及不止一类rRNA甲基化酶。发现许多菌株（n = 18）产生红霉素酯酶II型和rRNA甲基化酶。ereB和ermAM之间的物理连锁可能是基因共传播的原因。尽管它们是外源性的，ereB和ermAM已经在肠杆菌科的各个属中传播。
The distribution of nucleotide sequences related to ereA, ereB, and ermAM was studied by colony hybridization in 112 strains of members of the family Enterobacteriaceae that are highly resistant to erythromycin. The ereA and ereB genes encoding erythromycin esterases type I and II, respectively, were detected in strains inactivating the 14-membered macrolides erythromycin and oleandomycin. Because all 52 strains resisting these antibiotics by inactivation were detected by ereA (n = 23), ereB (n = 23), or both probes (n = 6), only two classes of genes accounted for this resistance phenotype. The ermAM gene encoding a streptococcal rRNA methylase was detected in 21 strains of Escherichia coli and two strains of Klebsiella spp. Determination of the MICs of macrolide, lincosamide, and streptogramin (MLS) antibiotics demonstrated a correlation between hybridization with ermAM and the so-called MLS resistance phenotype. The presence of 11 strains coresistant to MLS antibiotics that did not hypridize to the erm AM probe suggests that as in gram-positive organisms, MLS resistance in members of the family Enterobacteriaceae involves more than one class of rRNA methylase. Numerous strains (n = 18) were found to produce both an erythromycin esterase type II and an rRNA methylase. Physical linkage between ereB and ermAM may be rsponsible for the codissemination of the genes. Despite their exogenous origin, ereB and ermAM are already disseminated in various genera of the Enterobacteriaceae.