The effect of nanofiber alignment on the maturation of engineered meniscus constructs
The effect of nanofiber alignment on the maturation of engineered meniscus constructs
复制标题
DOI：
10.1016/j.biomaterials.2007.01.004
复制
发表时间：
2007-04-01
期刊：
影响因子：
14
通讯作者：
Mauck, Robert L.
中科院分区：
文献类型：
--
作者：
Baker, Brendon M.;Mauck, Robert L.
纤维软骨韧带是对膝关节的正常功能至关重要的承重组织。损伤后切除半月板损伤区域会损害膝关节功能，使患者易患骨关节炎。在这项研究中，我们采用可生物降解的纳米纤维支架半月板的组织工程。非对齐（NA）或纤维对齐（AL）的纳米纤维支架接种有椎间盘纤维软骨细胞（MFC）或间充质干细胞（MSC），以测试纤维对齐将增加基质含量和组织，从而改善机械性能的假设。此外，我们提出MSC可以作为MFC的替代品。随着培养时间的推移，MSC和MFC接种的NA和AL构建体的细胞结构和细胞外基质（ECM）含量增加。与我们最初的假设相反，NA和AL构建体含有相当量的ECM，尽管与接种有任一细胞类型的NA构建体相比，观察到AL的机械性质显著更大的增加。在10周内，细胞接种的NA构建体的模量增加了类似于IMPa，而细胞接种的AL构建体增加了> 7 MPa。此外，MSC-构建体产生了更大量的ECM，并表现出相当的机械性能的增加，从而证实了MSC用于半月板组织工程的效用。这些结果表明，细胞接种的纤维排列的纳米纤维支架可以作为指导组织生长的指导性微观模式，重建天然组织的形式和功能。(c)2007爱思唯尔有限公司版权所有。
The fibrocartilaginous menisci are load-bearing tissues vital to the normal functioning of the knee. Removal of damaged regions of the meniscus subsequent to injury impairs knee function and predisposes patients to osteoarthritis. In this study, we employed biodegradable nanofibrous scaffolds for the tissue engineering of the meniscus. Non-aligned (NA) or fiber-aligned (AL) nanofibrous scaffolds were seeded with meniscal fibrochondrocytes (MFCs) or mesenchymal stem cells (MSCs) to test the hypothesis that fiber-alignment would augment matrix content and organization, resulting in improved mechanical properties. Additionally, we proposed that MSCs could serve as an alternative to MFCs. With time in culture, MSC- and MFC-seeded NA and AL constructs increased in cellularity and extracellular matrix (ECM) content. Counter our initial hypothesis, NA and AL constructs contained comparable amounts of ECM, although a significantly larger increase in mechanical properties was observed for AL compared to NA constructs seeded with either cell type. Cell-seeded NA constructs increased in modulus by similar to 1 MPa over 10 weeks while cell-seeded AL construct increased by > 7 MPa. Additionally, MSC-constructs yielded greater amounts of ECM and demonstrated comparable increases in mechanical properties, thereby confirming the utility of MSCs for meniscus tissue engineering. These results demonstrate that cell-seeded fiber-aligned nanofibrous scaffolds may serve as an instructive micro-pattern for directed tissue growth, reconstituting both the form and function of the native tissue. (c) 2007 Elsevier Ltd. All rights reserved.