Semiautomated spectrophotometry of total phospholipids in plasma.
Semiautomated spectrophotometry of total phospholipids in plasma.
复制标题
血浆中总磷脂的半自动分光光度法。
DOI：
10.1093/clinchem/23.9.1766
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发表时间：
1977
影响因子：
9.3
通讯作者：
D. G. Wootton
中科院分区：
文献类型：
--
作者：
D. G. Wootton
本文报道了一种一步半自动测定血浆中总磷脂的方法。磷脂被提取到乙醇水中，与Lewis酸结合，并用比色法监测pH的变化。该方法与Gomori的酸消化法[J.Lab.克莱恩。地中海医院。27,955(1942)]，给出的结果大约低0.25 mmol/升，但似乎是一种合适的、危害较小的替代方案。
A one-stage semiautomated method for estimating total phospholipids in plasma is reported. The phospholipids are extracted into aqueous ethanol, combined with a Lewis acid, and the change in pH is monitored colorimetrically. The method, compared with the acid digestion method of Gomori [J. Lab. Clin. Med. 27, 955 (1942)], gives results that are about 0.25 mmol/liter lower, but seems to be a suitable and less hazardous alternative.