Generation and analysis of an IgG anti-platelet autoantibody reveals unusual molecular features.
Generation and analysis of an IgG anti-platelet autoantibody reveals unusual molecular features.
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IgG 抗血小板自身抗体的生成和分析揭示了不寻常的分子特征。
DOI：
10.1046/j.1365-2141.1997.d01-2112.x
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发表时间：
1997
影响因子：
6.5
通讯作者：
Chen,P
中科院分区：
文献类型：
--
作者：
Olee,T;En,J;Lai,CJ;Mo,L;Cho,CS;Wei,X;Wang,XF;WoodsJr,VL;Chen,P
Although serum transfer studies implicate IgG anti‐platelet autoantibodies in the premature platelet destruction of idiopathic thrombocytopenic purpura (ITP), many characteristics of these putative pathogenic autoantibodies remain unclear. The inability to obtain relevant monoclonal autoantibodies from patients has prevented their molecular, genetic and functional studies as a homogenous population. We have generated a monoclonal IgG anti‐platelet αIIbβ3autoantibody (termed G1) from an ITP patient. G1 binds human platelets (both resting and activated) and purified αIIbβ3with aKdof 1.57 × 10−8M. G1 utilizes VH4 and Vλ2 genes. The G1 VH region apparently has a 30 nucleotide insertion in its second complementarity determining region (CDR). Notably, somatic CDR insertion in the VH region has been observed only in one IgG rheumatoid factor, and not in any characterized polyreactive human autoantibodies reported in the literature. Combined, these data suggest G1 may be a disease‐relevant autoantibody. Further generation and study of monoclonal IgG anti‐platelet antibodies are warranted to determine the significance of such unusual autoantibodies in the immunopathogenesis of chronic ITP.
