Changes in polyamines, RNA synthesis, and cell proliferation during matrix-induced cartilage, bone, and bone marrow development.
Changes in polyamines, RNA synthesis, and cell proliferation during matrix-induced cartilage, bone, and bone marrow development.
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基质诱导的软骨、骨和骨髓发育过程中多胺、RNA 合成和细胞增殖的变化。
DOI：
10.1016/0012-1606(81)90446-2
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发表时间：
1981
影响因子：
2.7
通讯作者：
A. Reddi
中科院分区：
文献类型：
--
作者：
N. Rath;A. Reddi
研究了基质诱导的软骨内骨和骨髓发育过程中腐胺、亚精胺和精胺及其生物合成酶鸟氨酸脱羧酶（ODC）和S-腺苷甲硫氨酸脱羧酶（SAM-脱羧酶）水平的变化。核糖核酸（RNA）的合成进行了研究，[3 H]尿苷掺入到酸沉淀馏分。ODC和SAM-脱羧酶活性在基质植入后第3天和第8天和第9天表现出相似的峰值，并分别与软骨祖细胞和骨祖细胞增殖期间[3 H]胸苷掺入的相应峰值相关。与此相反，SAM-脱羧酶，但不是ODC的增加，在造血骨髓形成过程中观察到，而在这些细胞中的[3 H]胸苷的掺入仍然很高。所有三种多胺的浓度在第1天较低，在第11天骨生成期间上升至峰值，此后下降。腐胺，而不是亚精胺或精胺，也表现出较早的小，但广泛的高峰在第3-7天，在间充质细胞增殖和软骨分化。在软骨形成和骨形成的早期阶段，[3 H]尿苷掺入酸可沉淀材料中在第5天和第11天表现出两个峰。第11天腐胺、亚精胺和精胺的峰值水平与骨生成过程中RNA合成增加相一致。放射自显影定位的[3 H]胸苷表示刺激细胞增殖邻近的基质颗粒之前，软骨和成骨。第11天，成骨前体细胞倾向于与软骨溶解灶紧密贴壁。
Changes in the levels of putrescine, spermidine, and spermine and their biosynthetic enzymes, ornithine decarboxylase (ODC) andS-adenosylmethionine decarboxylase (SAM-decarboxylase) were studied during matrix-induced endochondral bone and bone marrow development. Ribonucleic acid (RNA) synthesis was studied by [3H]uridine incorporation into an acid-precipitable fraction. ODC and SAM-decarboxylase activity exhibited similar peaks on Day 3 and on Days 8 and 9 after matrix implantation and were correlated with corresponding peaks of [3H]thymidine incorporation during proliferation of chondroprogenitor and osteoprogenitor cells, respectively. In contrast an increase in SAM-decarboxylase but not ODC was observed during hematopoietic bone marrow formation while the incorporation of [3H]thymidine in these cells remained high. The concentrations of all three polyamines were low on Day 1 and rose to peak values during osteogenesis on Day 11 and declined thereafter. Putrescine, but not spermidine or spermine, also exhibited an earlier smaller but broad peak on Days 3–7 during mesenchymal cell proliferation and cartilage differentiation. Incorporation of [3H]uridine into acid-precipitable material exhibited two peaks on Days 5 and 11 during early phase of chondrogenesis and osteogenesis. The peak levels of putrescine, spermidine, and spermine on Day 11 coincided with increased RNA synthesis during osteogenesis. Autoradiographic localization of [3H]thymidine indicated a stimulation of cell proliferation adjacent to matrix particles prior to chondrogenesis and osteogenesis. Osteogenic precursor cells tended to be in close apposition to chondrolytic foci on Day 11.