Lung transit of 111Indium-labelled granulocytes. Relationship to labelling techniques.
Lung transit of 111Indium-labelled granulocytes. Relationship to labelling techniques.
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111 铟标记粒细胞的肺转运。
DOI：
10.1111/j.1600-0609.1983.tb01463.x
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
J. Lavender
中科院分区：
文献类型：
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作者：
S. Saverymuttu;A. Peters;H. Danpure;H. Reavy;S. Osman;J. Lavender
用伽马相机和计算机动态成像记录的111铟标记的粒细胞的早期体内分布因分离和标记技术的不同而不同。跟随静脉注射。团注，可确定4种动力学模式：(A)快速通过肺血管，(B)延迟通过肺，清除约30min，(C)在肺内完全滞留，最多10min，然后在1~2小时内缓慢释放，(D)延迟通过肺，时间与(B)相似，但随后肝脏大量摄取。用111In-tropolate标记并在整个标记过程中保持在血浆中的粒细胞，无论是以“纯”(通过血浆浓缩密度梯度离心法分离)还是“粗”(通过差速离心法分离)制剂注射，都表现出A型动力学，被认为最能代表粒细胞的正常行为。在生理盐水中用111In-乙酰丙酮标记的“粗”细胞表现为B型动力学。在Percoll-生理盐水中分离并用111In-乙酰丙酮标记的“纯”细胞显示C型动力学，被认为代表粒细胞‘刺激’和/或损伤，或D型动力学，被认为代表严重损伤。强调了用能使细胞行为改变最小的技术标记粒细胞用于动力学研究的重要性。
The early in vivo distribution of 111Indium-labelled granulocytes, recorded by dynamic imaging using a gamma camara and computer, varied according to the separation and labelling technique. Following i.v. bolus injection, 4 kinetic patterns could be identified: (A) rapid transit through the pulmonary vasculature, (B) delayed transit through the lung with clearance by about 30 min, (C) complete retention by the lung, for up to 10 min, followed by slow release over a period of 1 to 2 h, (D) delayed transit through the lung with a similar time course to (B) but with subsequent heavy liver uptake. Granulocytes labelled with 111In-tropolonate and maintained in plasma throughout the labelling procedure, whether injected as a 'pure' (separated by plasma-enriched density gradient centrifugation) or 'crude' (separated by differential centrifugation) preparation, displayed type A kinetics, thought to most closely represent the normal behaviour of granulocytes. 'Crude' cells labelled in saline with 111In-acetylacetonate displayed type B kinetics. 'Pure' cells isolated on Percoll-saline and labelled in saline with 111In-acetylacetonate displayed type C kinetics, thought to represent granulocyte 'stimulation' and/or damage, or type D kinetics, thought to represent severe damage. The importance is stressed of labelling granulocytes for kinetic studies with a technique that results in minimal alteration of cell behaviour.