Characterization of proanthocyanidins in pea (Pisum sativum L.), lentil (Lens culinaris L.), and faba bean (Vicia faba L.) seeds
Characterization of proanthocyanidins in pea (Pisum sativum L.), lentil (Lens culinaris L.), and faba bean (Vicia faba L.) seeds
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DOI：
10.1016/j.foodres.2011.11.018
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发表时间：
2012-05-01
影响因子：
8.1
通讯作者：
Reinecke, Dennis M.
中科院分区：
文献类型：
--
作者：
Jin, Alena (Lihua);Ozga, Jocelyn A.;Reinecke, Dennis M.
在豌豆（Pisum sativum L）、小扁豆（Lens culinaris L）和蚕豆（Vicia faba L）种子中鉴定了聚合类黄酮原花青素（PAs）。在过量间苯三酚（间苯三酚解）存在下进行酸催化后，采用反相高效液相色谱-光电二极管阵列检测法测定了PA亚基组成、定量和聚合度。采用液相色谱-质谱法对PA亚基进行鉴定。3种豆科植物均存在b型PAs；然而，这些豆科种子作物的PA亚基组成各不相同。在豌豆品种中，PA亚基组成几乎完全是prodelphinidin（没食子儿茶素和表没食子儿茶素）。蚕豆和扁豆种子中含有丰富的原花青素型和原飞蓟素型黄烷-3-醇亚基。这些豆科谷物的种子PA含量约为270至650毫克/100克鲜重。这些数据表明，特定品种的豌豆、蚕豆和扁豆的种子可以成为原花青素的良好膳食来源。(c) 2011 Elsevier Ltd.版权所有。
The polymeric flavonoids, proanthocyanidins (PAs), were characterized in seeds of the grain legumes of pea (Pisum sativum L), lentil (Lens culinaris L), and faba bean (Vicia faba L). PA subunit composition, quantitation and degree of polymerization were determined using reversed-phase high performance liquid chromatography-photodiode array detection after acid-catalysis in the presence of excess phloroglucinol (phloroglucinolysis). Liquid chromatography-mass spectrometry was used to confirm PA subunit identity. B-type PAs were found in all three grain legume species; however, the PA subunit composition varied among these legume seed crops. In the pea cultivars, the PA subunit composition was almost exclusively prodelphinidin (gallocatechin and epigallocatechin). Both procyanidin- and prodelphinidin-type flavan-3-ol subunits were abundant in the PAs of faba bean and lentil seeds tested. The seed PA content of these grain legumes ranged from approximately 270 to 650 mg/100 g fresh weight. These data show that seeds of specific cultivars of pea, faba bean and lentil can be a good dietary source of proanthocyanidins. (c) 2011 Elsevier Ltd. All rights reserved.