Dextran sodium sulfate enhances secretion of recombinant human transferrin in Schizosaccharomyces pombe
Dextran sodium sulfate enhances secretion of recombinant human transferrin in Schizosaccharomyces pombe
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DOI：
10.1007/s00253-009-2130-5
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发表时间：
2009-11-01
影响因子：
5
通讯作者：
Takegawa, Kaoru
中科院分区：
文献类型：
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作者：
Mukaiyama, Hiroyuki;Giga-Hama, Yuko;Takegawa, Kaoru
研究了培养基添加对裂殖酵母裂殖酵母异源生产人血清转铁蛋白（hTF）的影响。野生型S.粟酒裂殖酵母细胞为了克服这种障碍，筛选培养基补充剂改善hTF分泌的能力。酪蛋白氨基酸（CAA），这已被报道，以增加异源蛋白的生产力在毕赤酵母，提高分泌hTF超过四倍。阴离子表面活性剂脱氧胆酸盐或聚乙二醇也能提高hTF的分泌。有趣的是，葡聚糖硫酸钠（DSS），一种聚阴离子表面活性剂，被发现比任何其他测试的补充剂更好地增强分泌的hTF的产生。在2%CAA存在下添加DSS对增加hTF分泌表现出协同作用，导致相对于常规条件增加约7倍。没有发现细胞生长受到DSS或CAA的添加的影响。DSS可以作为表面活性剂，也可以促进脂质体的锚定，这些特性可以有助于通过质膜的有效分泌或胞吐。
The effect of medium supplementation on heterologous production of human serum transferrin (hTF) in the fission yeast Schizosaccharomyces pombe has been investigated. The productivity of recombinant hTF was low in wild-type S. pombe cells. To overcome this impediment, culture media supplements were screened for their ability to improve secretion of hTF. Casamino acids (CAA), which have been reported to increase heterologous protein productivity in Pichia pastoris, improved the secretion hTF by more than fourfold. An anion surfactant deoxycholate or polyethylene glycol also improved the secretion hTF. Interestingly, dextran sodium sulfate (DSS), a poly-anion surfactant, was found to enhance production of secreted hTF better than any other supplement tested. Addition of DSS in the presence of 2% CAA exhibited a synergistic effect on increasing hTF secretion, resulting in an increase of about sevenfold relative to conventional conditions. Cell growth was not found to be affected by the addition of DSS or CAA. DSS may act as a surfactant and may also facilitate the anchoring of liposomes, and these properties may contribute to efficient secretion or exocytosis through the plasma membrane.