Overexpression of angiopoietin-1 and angiopoietin-2 in hepatocellular carcinoma
Overexpression of angiopoietin-1 and angiopoietin-2 in hepatocellular carcinoma
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DOI：
10.1016/j.jhep.2004.01.027
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发表时间：
2004-05-01
影响因子：
25.7
通讯作者：
Kojiro, M
中科院分区：
文献类型：
--
作者：
Torimura, T;Ueno, T;Kojiro, M
背景/目的：肝细胞癌（HCC）是一种高度血管化的肿瘤。血管生成素-1和血管生成素-2已被证明参与肿瘤血管生成。方法：采用实时荧光定量PCR方法检测缺氧条件下培养的肝癌细胞、肝癌组织和非癌肝组织中Angiopoietin-1和Angiopoietin-2 mRNA的表达。采用免疫组化法检测Angiopoietin-1、Angiopoietin-2及其受体Tie-2在HCC中的表达。结果：低氧条件下，肝癌细胞中Angiopoietin-1和Angiopoietin-2 mRNA的表达水平没有明显上调，而Angiopoietin-1和Angiopoietin-2 mRNA的表达水平与肿瘤分化程度和肿瘤血管形成的关系没有明显的差异。在HCC中检测到Angiopoietin-1和Angiopoietin-2 mRNA的表达增加。在肝癌细胞、肝星状细胞和平滑肌细胞中检测到血管生成素-1和血管生成素-2，而在内皮细胞、肝星状细胞和平滑肌细胞中检测到Tie-2。Angiopoietin-2和Angiopoietin-2 mRNA表达的增加与肿瘤去分化有关。Angiopoietin-1和Angiopoietin-2的表达与HCC血管生成密切相关。结论：Angiopoietin-1和Angiopoietin-2的高表达在HCC血管生成过程中起重要作用。(C)2004年欧洲肝脏研究协会。Elsevier B. V.出版，保留所有权利。
Background/Aims: Hepatocellular carcinoma (HCC) is a highly vascular tumor. Angiopoietin-1 and Angiopoietin-2 have been shown to be involved in tumor angiogenesis. We investigated the expression of Angiopoietin-1 and Angiopoietin-2 in HCC.Methods: The expression of Angiopoietin-1 and Angiopoietin-2 mRNAs in cultured hepatoma cells under hypoxic conditions and in HCC and noncancerous liver tissue was evaluated by real-time PCR. The expression of Angiopoietin-1, Angiopoietin-2, and their receptor Tie-2 in HCC was assessed by immunohistochemistry. The changes in Angiopoietin-1 and Angiopoietin-2 expression were evaluated in relation to tumor differentiation and changes in tumor vascularity.Results: Hypoxic conditions did not up-regulate the expression of Angiopoietin-1 and Angiopoietin-2 mRNAs in hepatoma cells. Increased expression of Angiopoietin-1 and Angiopoietin-2 mRNAs was detected in HCC. Angiopoietin-1 and Angiopoietin-2 were detected in hepatoma cells, hepatic stellate cells, and smooth muscle cells, whereas Tie-2 was detected in endothelial cells, hepatic stellate cells and smooth muscle cells. Increased expression of Angiopoietin-2 and Angiopoietin-2 mRNA was associated with tumor dedifferentiation. The expression of Angiopoietin-1 and Angiopoietin-2 correlated with HCC vascularity.Conclusions: Our findings indicate that the increased expression of Angiopoietin-1 and Angiopoietin-2 play a critical role in the process of vascular development in HCC. (C) 2004 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.