Digital transcriptome analysis indicates adaptive mechanisms in the heart of a hibernating mammal
Digital transcriptome analysis indicates adaptive mechanisms in the heart of a hibernating mammal
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DOI：
10.1152/physiolgenomics.00076.2005
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发表时间：
2005-10-17
影响因子：
4.6
通讯作者：
Andrews, MT
中科院分区：
文献类型：
--
作者：
Brauch, KM;Dhruv, ND;Andrews, MT
在冬眠期间，接近冰点的体温和减少的血液流动可能是特定基因表达变化的结果。在这项研究中，我们描述了一个全面的调查基因在心脏的十三线地松鼠（Spermophilus tridecemlineatus）之前和冬眠期间。选择心脏进行这项研究是因为它是一个收缩器官，尽管体温为5 ℃，并且在5-6个月的时间内缺乏食物，但它必须继续工作。我们使用了一种数字化基因表达检测方法，包括对来自活跃和冬眠地松鼠心脏的定向cDNA文库进行高通量测序，以确定3，532个表达序列标签（EST）的身份和频率。对活动期和冬眠期心脏表达谱的统计分析表明，基于P
Survival of near-freezing body temperatures and reduced blood flow during hibernation is likely the result of changes in the expression of specific genes. In this study, we described a comprehensive survey of mRNAs in the heart of the thirteen-lined ground squirrel (Spermophilus tridecemlineatus) before and during hibernation. The heart was chosen for this study because it is a contractile organ that must continue to work despite body temperatures of 5 C and the lack of food for periods of 5-6 mo. We used a digital gene expression assay involving high-throughput sequencing of directional cDNA libraries from hearts of active and hibernating ground squirrels to determine the identity and frequency of 3,532 expressed sequence tags (ESTs). Statistical analysis of the active and hibernating heart expression profile indicated the differential regulation of 48 genes based on a P