Viperidae Snake Venoms Block Nicotinic Acetylcholine Receptors and Voltage-Gated Ca2+ Channels in Identified Neurons of Fresh-Water Snail Lymnaea stagnalis
Viperidae Snake Venoms Block Nicotinic Acetylcholine Receptors and Voltage-Gated Ca2+ Channels in Identified Neurons of Fresh-Water Snail Lymnaea stagnalis
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DOI：
10.1134/s1990747808010030
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发表时间：
2008-03-01
影响因子：
0.5
通讯作者：
Vulfius, C. A.
中科院分区：
文献类型：
--
作者：
Gorbacheva, E. V.;Starkov, V. G.;Vulfius, C. A.
蛇毒含有大量的有毒多肽成分，与各种细胞靶点相互作用。因此，Elapidae蛇毒含有对烟碱乙酰胆碱受体（nAChR）具有非常高亲和力的α-神经毒素和能够抑制Ca 2+和K+通道活性的少数毒素。蝰蛇科蛇毒液中存在nAChR拮抗剂和电压门控离子通道阻滞剂的实验证据非常缺乏。本研究首次研究了7种蛇类（蝰蛇Vipera nikolskii，Echis multisquamatus，Gloydius saxatilis，Bitis arietans，蝰蛇Vipera renardi，蝰蛇Vipera lebetina，眼镜蛇Naja kaouthia）粗毒对nAChRs和电压门控Ca ~（2+）通道的影响。采用电压钳和细胞内灌流技术，对淡水软体动物滞水沙蚕的神经元进行了分离鉴定。研究中的所有蝰蛇毒液都阻断了nAChRs和电压门控性Ca 2+通道。与N.而蝰蛇科蛇毒和N. kaouthia蛇毒对Ca ~（2+）通道的阻断程度则不同。Kaouthia是相似的。所获得的数据表明，在这项研究中测试的蝰蛇蛇毒含有肽与nAChRs和电压门控钙通道的亲和力。
Snake venoms contain a vast array of toxic polypeptide components interacting with a variety of cell targets. Thus, Elapidae snake venoms contain a -neurotoxins with very high affinity for nicotinic acetylcholine receptors (nAChRs) and a few toxins able to suppress the activity of Ca2+ and K+ channels. Experimental evidence for the presence of nAChR antagonists and voltage-gated ionic channel blockers in venoms of Viperidae snakes is very scarce. In this study, effects of crude venoms of seven snake species (Vipera nikolskii, Echis multisquamatus, Gloydius saxatilis, Bitis arietans, Vipera renardi, Vipera lebetina, and Naja kaouthia) on nAChRs and voltage-gated Ca2+ channels were studied for the first time. The experiments were carried out on isolated identified neurons of the fresh-water mollusc Lymnaea stagnalis using voltage-clamp and intracellular perfusion techniques. All Viperidae snake venoms under study blocked nAChRs and voltage-gated Ca2+ channels. The potency of these venoms against nAChRs was significantly lower in comparison with N. kaouthia venom which is rich of a -neurotoxins; however, the extent of Ca2+ channel block by venoms of Viperidae snakes and N. kaouthia was similar. The data obtained suggest that Viperidae snake venoms tested in this study contain peptides with affinity both for nAChRs and for voltage-gated Ca2+ channels.