Heat-stress effects on the myosin heavy chain phenotype of rat soleus fibers during the early stages of regeneration
Heat-stress effects on the myosin heavy chain phenotype of rat soleus fibers during the early stages of regeneration
复制标题
再生早期阶段热应激对大鼠比目鱼肌纤维肌球蛋白重链表型的影响
DOI：
10.1002/mus.24686
复制
发表时间：
2015
期刊：
影响因子：
3.4
通讯作者：
Ohira Y.
中科院分区：
文献类型：
--
作者：
Oishi Y;Roy RR;Ogata T;Ohira Y.
简介：我们研究了热应激对再生早期比目鱼肌纤维成年肌球蛋白重链（MyHC）谱的影响。方法：观察布比卡因注射后2、4、6天成年大鼠的纤维再生情况。布比卡因注射后24小时，大鼠在42±1℃的水中浸泡30分钟，此后每隔一天进行热应激。结果：2天后未见成体MyHC亚型，4天后部分纤维仅表达快速MyHC亚型。热应激6天后，再生纤维中MyHC升高快、慢。仅在热应激肌肉中观察到表达缓慢MyHC的再生纤维。布比卡因注射增加了再生纤维中Pax7+和MyoD+卫星细胞的数量，在热应激大鼠中更明显。结论：结果表明，热应激通过激活卫星细胞加速再生纤维中MyHC表型从快到慢的转化。中国生物医学工程学报（英文版），2015
Introduction:We investigated heat‐stress effects on the adult myosin heavy chain (MyHC) profile of soleus muscle fibers at an early stage of regeneration.Methods:Regenerating fibers in adult rats were analyzed 2, 4, or 6 days after bupivacaine injection. Rats were heat stressed by immersion in water (42 ± 1°C) for 30 minutes 24 hours after bupivacaine injection and every other day thereafter.Results:No adult MyHC isoforms were observed after 2 days, whereas some fibers expressed only fast MyHC after 4 days. Heat stress increased fast and slow MyHC in regenerating fibers after 6 days. Regenerating fibers expressing only slow MyHC were observed only in heat‐stressed muscles. Bupivacaine injection increased the number of Pax7+and MyoD+satellite cells in regenerating fibers, more so in heat‐stressed rats.Conclusion:The results indicate that heat stress accelerates fast‐to‐slow MyHC phenotype conversion in regenerating fibers via activation of satellite cells.Muscle Nerve52: 1047–1056, 2015