Metabolism of endogenous surfactant in premature baboons and effect of prenatal corticosteroids.
Metabolism of endogenous surfactant in premature baboons and effect of prenatal corticosteroids.
复制标题
早产狒狒内源性表面活性剂的代谢及产前皮质类固醇的作用。
DOI：
10.1164/ajrccm.160.5.9808070
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Zimmermann,LJ
中科院分区：
文献类型：
--
作者：
Bunt,JE;Carnielli,VP;Seidner,SR;Ikegami,M;DarcosWattimena,JL;Sauer,PJ;Jobe,AH;Zimmermann,LJ
我们在极早产狒狒体内研究了表面活性剂的合成和产前倍他米松治疗的效果。 10 只怀孕狒狒被随机分配，在早产前 48 小时和 24 小时接受倍他米松（β）或盐水（对照）。新生狒狒插管、表面活性剂处理、通气6 d。他们接受了 24 小时的稳定同位素 [U-13C] 葡萄糖输注，作为在表面活性剂磷脂酰胆碱 (PC) 中合成棕榈酸的前体。表面活性剂 PC 中的棕榈酸在同位素输注开始后 27 ± 2 小时开始富集，并在 100 ± 4 小时时达到最大富集。 β 组中 PC 棕榈酸酯的分数合成率 (1.5 ± 0.2%/d) 比对照 (0.7 ± 0.1%/d) 增加了 129%（p < 0.02，Mann-Whitney U 检验）。 β 组中 PC 的绝对合成率 [1.6 ± 0.3 μ mol/kg/d] 比对照组 [0.7 ± 0.2 μ mol/kg/d] 增加了 128%（p < 0.02）。这些数据表明，以血浆葡萄糖为前体合成内源性表面活性剂是一个缓慢的过程。首次在体内证明，产前糖皮质激素可刺激早产狒狒体内表面活性剂 PC 的合成。 Bunt JEH、Carnielli VP、Seidner SR、Ikegami M、Wattimena JLD、Sauer PJJ、Jobe AH、Zimmermann LJI。早产狒狒内源性表面活性剂的代谢及产前皮质类固醇的作用。
We studied the synthesis of surfactant and the effect of prenatal betamethasone treatmentin vivoin very preterm baboons. Ten pregnant baboons were randomized to receive either betamethasone (beta) or saline (control) 48 and 24 h before preterm delivery. The newborn baboons were intubated, treated with surfactant, and ventilated for 6 d. They received a 24-h infusion with the stable isotope [U-13C]glucose as precursor for the synthesis of palmitic acid in surfactant phosphatidylcholine (PC). Palmitic acid in surfactant PC became enriched 27 ± 2 h after the start of the isotope infusion and was maximally enriched at 100 ± 4 h. The fractional synthesis rate of PC palmitate in the beta group (1.5 ± 0.2%/d) was increased by 129% above control (0.7 ± 0.1%/d) (p < 0.02, Mann– Whitney U test). The absolute synthesis rate of PC in the beta group [1.6 ± 0.3 μ mol/kg/d] was increased by 128% above controls [0.7 ± 0.2 μ mol/kg/d] (p < 0.02). These data show that the synthesis of endogenous surfactant from plasma glucose as precursor is a slow process. It is shown, for the first timein vivo, that prenatal glucocorticosteroids stimulate the synthesis of surfactant PC in the very premature baboon. Bunt JEH, Carnielli VP, Seidner SR, Ikegami M, Wattimena JLD, Sauer PJJ, Jobe AH, Zimmermann LJI. Metabolism of endogenous surfactant in premature baboons and effect of prenatal corticosteroids.