Mie theory interpretations of light scattering from intact cells.
Mie theory interpretations of light scattering from intact cells.
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DOI：
10.1364/ol.30.002442
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发表时间：
2005-09
期刊：
影响因子：
3.6
通讯作者：
Jeremy D. Wilson;T. Foster
中科院分区：
文献类型：
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作者：
Jeremy D. Wilson;T. Foster
我们提出了基于米氏理论的完整细胞角分辨光散射分析，其中涉及粒径分布 rho 和散射截面 sigma 的乘积。分析表明，确定细胞内细胞器大小的能力很大程度上取决于粒径分布的假定功能形式；然而，无论这种选择如何，产品 sigma rho 都会提取主要的光散射体。我们检查了我们自己和另一个实验室的测角仪测量结果，发现该分析协调了看似相互矛盾的结果，并证实线粒体大小的细胞器在 50 度 - 90 度的角度范围内主导完整细胞的散射。
We present a Mie theory-based analysis of angularly resolved light scattering from intact cells that involves the product of a particle size distribution rho and the scattering cross section sigma. The analysis shows that the ability to size intracellular organelles depends strongly upon the assumed functional form of the particle size distribution; however, the product sigma rho extracts the dominant light scatterers regardless of this choice. We examine goniometer measurements from our own and another laboratory and find that this analysis reconciles seemingly conflicting results and confirms that mitochondrial-sized organelles dominate scattering from intact cells in the angular range 50 degrees - 90 degrees.