Improved detection of apoptotic cells in archival paraffin sections: Immunohistochemistry using antibodies to cleaved caspase 3
Improved detection of apoptotic cells in archival paraffin sections: Immunohistochemistry using antibodies to cleaved caspase 3
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DOI：
10.1177/002215540205000401
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发表时间：
2002-04-01
影响因子：
3.2
通讯作者：
Willingham, MC
中科院分区：
文献类型：
--
作者：
Gown, AM;Willingham, MC
凋亡在许多生物学过程的研究中已变得至关重要，包括肿瘤形成、神经退行性疾病和发育。许多研究的局限之一是难以特异性地识别单个凋亡细胞。在众多已开发的检测凋亡细胞的特定方法中，大多数不适用于存档的石蜡包埋组织的组织学切片。最近，对凋亡分子事件理解的进展使人们认识到，半胱天冬酶激活是迄今为止这种细胞自杀机制最特异的指标。一些出版物报道了针对新表位的抗体的开发，这些新表位是通过半胱天冬酶的作用在各种底物中产生的。其中之一是存在于活化的半胱天冬酶3上的新表位，半胱天冬酶3是一种广泛分布的半胱天冬酶，是细胞内凋亡级联反应的主要效应半胱天冬酶。这项研究证明了在存档石蜡切片中使用一种最近市售的针对裂解的半胱天冬酶3的抗体的实用性，表明这可能是一种高度特异且敏感的方法，通常适用于许多存档材料的研究。
Apoptosis has gained central importance in the study of many biological processes, including neoplasia, neurodegenerative diseases, and development. One of the limitations of many studies is the difficulty of specifically identifying individual apoptotic cells. Of the many specific methods developed to detect apoptotic cells, most are not applicable to histological sections of archival paraffin-em bedded tissues. Recently, advances in the understanding of the molecular events in apoptosis have led to the realization that caspase activation is by far the most specific indicator of this cell suicide mechanism. Several publications have reported the development of antibodies directed at neoepitopes that are generated in various substrates through the action of caspases. One of these is that present on activated caspase 3, a ubiquitously distributed caspase that is a main effector caspase of the apoptotic cascade within cells. This study demonstrates the utility of using a recently commercially available antibody to cleaved caspase 3 in archival paraffin sections, suggesting that this may be a highly specific and sensitive method generally applicable to many studies of archival material.