Respiratory epithelium inhibits bronchial smooth muscle tone.
Respiratory epithelium inhibits bronchial smooth muscle tone.
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呼吸道上皮抑制支气管平滑肌张力。
DOI：
10.1152/jappl.1985.58.3.834
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Vanhoutte,PM
中科院分区：
文献类型：
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作者：
Flavahan,NA;Aarhus,LL;Rimele,TJ;Vanhoutte,PM
本研究的目的是确定呼吸上皮是否可以调节支气管平滑肌的反应性。将犬支气管（4-6 mm OD）的成对环（其中一些已机械去除上皮（通过摩擦管腔表面））固定在生理盐水溶液中，用95% O2-5% CO2充气，并保持在37 ℃下。通过组织学检查确认上皮的存在或不存在。去除上皮增加了乙酰胆碱，组胺和5-羟色胺引起的收缩反应。在上皮存在和不存在的情况下，神经刺激诱发了类似的峰值反应。在未摩擦的制剂中，当刺激继续时，峰值反应随后逐渐降低。这种下降，持续存在的普萘洛尔，没有观察到上皮剥脱制剂。在支气管环收缩与乙酰胆碱，异丙肾上腺素产生浓度依赖性松弛，这是显着更大的环与上皮相比，裸露的环。这些结果表明，呼吸道上皮细胞可能产生抑制信号，以降低支气管平滑肌收缩激动剂的反应性，并增加抑制性刺激的有效性。
The aim of the present study was to determine whether or not the respiratory epithelium can modulate the responsiveness of bronchial smooth muscle. Paired rings of canine bronchi (4–6 mm OD), in some of which the epithelium had been removed mechanically (by rubbing the luminal surface), were mounted in physiological saline solution, gassed with 95% O2–5% CO2, and maintained at 37 degrees C. The presence or absence of the epithelium was confirmed by histological examination. Removal of the epithelium increased the contractile responses evoked by acetylcholine, histamine, and 5-hydroxytryptamine. Transmural nerve stimulation evoked similar peak responses in the presence and absence of epithelium. In unrubbed preparations, the peak response was followed by a gradual decrease when the stimulation was continued. This decrease, which persisted in the presence of propranolol, was not observed in epithelium-denuded preparations. In bronchial rings contracted with acetylcholine, isoproterenol produced concentration-dependent relaxations which were significantly greater in rings with epithelium compared with denuded rings. These results suggest that respiratory epithelial cells may generate an inhibitory signal to decrease the responsiveness of bronchial smooth muscle to contractile agonists and augment the effectiveness of inhibitory stimuli.