Characterization of Antilymphocytic Antibody
Characterization of Antilymphocytic Antibody
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抗淋巴细胞抗体的表征
作者：
Keith James;Peter Medawar
现在众所周知，抗淋巴细胞血清 (ALS) 具有延长注射该血清的动物同种移植物寿命的功效1-3。所有研究过 ALS 的人都同意，其免疫抑制能力的很大一部分在于 7S 球蛋白部分2。这里描述的实验代表了对在兔子和马中饲养的不同部分的 ALS 延长 CBA 小鼠 A 品系尾部皮肤同种移植物的寿命的能力的正式分析。采用Levey和Medawar3的方法制备兔抗小鼠胸腺细胞抗血清。马抗血清是由相同技术的放大变体制成的，由威康基金会慷慨地提供给我们。通常将血清级分重构至它们最初存在于全血清中的浓度，并通过皮下注射0.5ml进行测定。植皮后第二天和第五天重复3次。
ANTILYMPHOCYTIC serum (ALS) is now well known to have the power of prolonging the life of homografts on the animals into which it is injected1–3. All who have studied ALS agree that a high proportion of its immunosuppressive power resides in the 7S globulin fraction2. The experiments described here represent a formal analysis of the power of different fractions of ALS raised in rabbits and horses to prolong the life of A-strain tail skin homo-grafts on CBA mice. Rabbit antisera against mouse thymocytes were prepared by the method of Levey and Medawar3. The horse antiserum, made by a scaled-up variant of the same technique, was kindly supplied to us by the Wellcome Foundation. The serum fractions were normally reconstituted to the concentrations at which they were originally present in whole serum, and assayed by the subcutaneous injection of 0.5 ml. on the second and again on the fifth days after skin grafting3.