Antineutrophil cytoplasmic antibodies against myeloperoxidase, proteinase 3, elastase, cathepsin G and lactoferrin in Japanese patients with rheumatoid arthritis
Antineutrophil cytoplasmic antibodies against myeloperoxidase, proteinase 3, elastase, cathepsin G and lactoferrin in Japanese patients with rheumatoid arthritis
复制标题
DOI：
10.3109/s10165-010-0356-9
复制
发表时间：
2011-02
影响因子：
2.2
通讯作者：
I. Kida;Shigeto Kobayashi;K. Takeuchi;H. Tsuda;H. Hashimoto;Y. Takasaki
中科院分区：
文献类型：
--
作者：
I. Kida;Shigeto Kobayashi;K. Takeuchi;H. Tsuda;H. Hashimoto;Y. Takasaki
测定抗髓过氧化物酶（MPO）、蛋白酶3（PR-3）、乳铁蛋白（LF）、组织蛋白酶G（CG）和弹性蛋白酶（EL）的抗神经细胞胞浆抗体（ANCA），以研究ANCA的存在是否与日本类风湿关节炎（RA）患者的关节外表现密切相关。本文用酶联免疫吸附试验（ELISA）检测了125例类风湿关节炎（RA）和83例其他风湿性疾病患者血清中的MPO、PR-3、LF、CG和EL抗体。根据病历研究患者的临床表现和实验室参数。125例RA患者中有30例（24.0%）ANCA阳性，至少有一种ANCA抗原。在5种ANCA中，抗LF抗体（抗LF）在RA患者中最常见（16.8%）。ANCA阳性RA患者的关节评分（JS）和ESR均高于ANCA阴性患者（分别为40.8 ± 43.3，24.3 ± 26.2，44.4 ± 22.4，28.9 ± 23.6，p < 0.05）。ANCA阳性和ANCA阴性患者间质性肺炎、皮肤血管炎、类风湿性结节和多发性单神经病的发生率无统计学差异。预期抗LF的存在具有病理学相关性，因为抗LF对LF的作用导致LF的抗炎活性的抑制。
Antineutrophil cytoplasmic antibodies (ANCAs) against myeloperoxidase (MPO), proteinase 3 (PR-3), lactoferrin (LF), cathepsin G (CG) and elastase (EL) were determined to investigate whether the presence of ANCAs is closely related to extra-articular manifestations in Japanese patients with rheumatoid arthritis (RA). Antibodies against MPO, PR-3, LF, CG and EL were determined in sera from 125 patients with RA and 83 sera from patients with other rheumatic diseases by enzyme-linked immunosorbent assay. Clinical manifestations and laboratory parameters of the patients were studied from medical records. Thirty of the 125 (24.0%) RA patients were positive for ANCAs for at least one of these 5 ANCA antigens. Among the 5 ANCAs, anti-LF antibody (anti-LF) (16.8%) was most commonly observed in patients with RA. A higher joint score (JS) and an elevated ESR were demonstrated in ANCA-positive RA patients compared to those of ANCA-negative patients (40.8 ± 43.3, 24.3 ± 26.2, p < 0.05, 44.4 ± 22.4, 28.9 ± 23.6, p < 0.05, respectively). No statistical differences in the presence of interstitial pneumonia, cutaneous vasculitis, rheumatoid nodules and mononeuropathy multiplex were observed between ANCA-positive and ANCA-negative patients. The presence of anti-LF is expected to be of pathological relevance, as the action of anti-LF towards LF results in the inhibition of the anti-inflammatory activity of LF.